概述

  • 产品名称Anti-Claudin 6抗体
    参阅全部 Claudin 6 一抗
  • 描述
    兔多克隆抗体to Claudin 6
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of human Claudin 6.

  • 阳性对照
    • human ovarian tissue, HUVEC cells, extracts from Jurkat cells.

性能

应用

Our Abpromise guarantee covers the use of ab75055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Plays a major role in tight junction-specific obliteration of the intercellular space.
  • 序列相似性Belongs to the claudin family.
  • 细胞定位Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Claudin-6 antibody
    • Claudin6 antibody
    • CLD6_HUMAN antibody
    • CLDN 6 antibody
    • CLDN6 antibody
    • OTTHUMP00000159248 antibody
    • Skullin 2 antibody
    • Skullin antibody
    • UNQ757/PRO1488 antibody
    see all

Anti-Claudin 6 antibody 图像

  • Human ovarian tissue with ab75055 at 1/50 dilution, in the absence and presence of the immunising peptide.
  • Huvec cells with ab75055 at 1/500 dilution, in the absence and presence of the immunising peptide.
  • All lanes : Anti-Claudin 6 antibody (ab75055) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells
    Lane 2 : extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <19 kDa. We are unsure as to the identity of these extra bands.

Anti-Claudin 6 antibody (ab75055)参考文献

This product has been referenced in:
  • Florimond A  et al. Human hepatic stellate cells are not permissive for hepatitis C virus entry and replication. Gut N/A:N/A (2014). Human . Read more (PubMed: 25063678) »
  • Sarhan MA  et al. Differential expression of candidate virus receptors in human T lymphocytes prone or resistant to infection with patient-derived hepatitis C virus. PLoS One 8:e62159 (2013). WB ; Human . Read more (PubMed: 23626783) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6 25C 100C 20 min
Sample Human Tissue sections (small intestine)
Specification small intestine
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
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Abcam user community

Verified customer

提交于 Aug 07 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Pancreas)
Specification Pancreas
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"