概述

  • 产品名称Anti-Claudin 5抗体
    参阅全部 Claudin 5 一抗
  • 描述
    兔多克隆抗体to Claudin 5
  • 经测试应用适用于: ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • 阳性对照
    • Lung

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab15106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 22547149
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
IHC-P 1/50.

靶标

  • 功能Plays a major role in tight junction-specific obliteration of the intercellular space.
  • 序列相似性Belongs to the claudin family.
  • 细胞定位Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Androgen withdrawal and apoptosis induced protein RVP1 like antibody
    • AWAL antibody
    • BEC 1 antibody
    • BEC1 antibody
    • Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody
    • Claudin-5 antibody
    • Claudin5 antibody
    • CLD5_HUMAN antibody
    • CLDN 5 antibody
    • Cldn5 antibody
    • CPETR L1 antibody
    • CPETRL 1 antibody
    • CPETRL1 antibody
    • TMDVCF antibody
    • TMVCF antibody
    • Transmembrane protein deleted in VCFS antibody
    • Transmembrane protein deleted in velocardiofacial syndrome antibody
    see all

Anti-Claudin 5 antibody 图像

  • ab15106 staining Claudin 5 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1.5% serum for 1 hour at 37°C; antigen retrieval was by heat mediation in a acetic acid. Samples were incubated with primary antibody (1/200 in blocking buffer) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

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  • ICC/IF image of ab15106 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15106, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab15106 staining Claudin 5 in human lung by Immunohistochemistry (FFPE-sections).

  • ab15106 staining Claudin 5 in the Human HBMEC cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton in PBS and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary antibody.

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  • Anti-Claudin 5 antibody (ab15106) at 1/1000 dilution + Mouse lung tissue lysate at 30 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 25°C.

    Incubated for 15 hours at 4°C with the primary antibody.

    See Abreview

Anti-Claudin 5 antibody (ab15106)参考文献

This product has been referenced in:
  • Kudinov AE  et al. Musashi-2 (MSI2) supports TGF-ß signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis. Proc Natl Acad Sci U S A 113:6955-60 (2016). WB . Read more (PubMed: 27274057) »
  • Kenesei K  et al. Enhanced detection with spectral imaging fluorescence microscopy reveals tissue- and cell-type-specific compartmentalization of surface-modified polystyrene nanoparticles. J Nanobiotechnology 14:55 (2016). Read more (PubMed: 27388915) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (neuroblastoma)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification neuroblastoma
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Apr 05 2017

Application Western blot
Sample Human Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Apr 05 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Cow Cell lysate - whole cell (Lung)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Lung
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Apr 05 2017

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Application Western blot
Sample African green monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Apr 05 2017

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Application Immunocytochemistry/ Immunofluorescence
Sample African green monkey Cell (Kidney)
Permeabilization Yes - 0.1% Troton X-100
Specification Kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
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提交于 Mar 24 2017

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.3% Troton X-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
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提交于 Jan 19 2017

Application Immunocytochemistry
Sample Mouse Cell (bEnd.3)
Permeabilization Yes - 0.1% Triton in PBS
Specification bEnd.3
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
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提交于 Nov 25 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HBMEC)
Permeabilization Yes - 0.1% Triton in PBS
Specification HBMEC
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
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提交于 Nov 25 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (15%)
Sample Mouse Tissue lysate - whole (lung)
Specification lung
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 08 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 37°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: acetic acid
Sample Mouse Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Paraformaldehyde
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提交于 Jul 30 2014

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