Androgen withdrawal and apoptosis induced protein RVP1 like antibody
BEC 1 antibody
Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody
CLDN 5 antibody
CPETR L1 antibody
CPETRL 1 antibody
Transmembrane protein deleted in VCFS antibody
Transmembrane protein deleted in velocardiofacial syndrome antibody
Anti-Claudin 5 antibody 图像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 5 antibody (ab15106)Image is courtesy of an anonymous AbReview
ab15106 staining Claudin 5 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1.5% serum for 1 hour at 37°C; antigen retrieval was by heat mediation in a acetic acid. Samples were incubated with primary antibody (1/200 in blocking buffer) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
ICC/IF image of ab15106 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15106, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab15106 staining Claudin 5 in human lung by Immunohistochemistry (FFPE-sections).
Immunocytochemistry/ Immunofluorescence - Anti-Claudin 5 antibody (ab15106)Image is courtesy of an anonymous AbReview
ab15106 staining Claudin 5 in the Human HBMEC cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton in PBS and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary antibody.
Kudinov AE et al. Musashi-2 (MSI2) supports TGF-ß signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis. Proc Natl Acad Sci U S A113:6955-60 (2016).
Read more (PubMed: 27274057) »
Kenesei K et al. Enhanced detection with spectral imaging fluorescence microscopy reveals tissue- and cell-type-specific compartmentalization of surface-modified polystyrene nanoparticles. J Nanobiotechnology14:55 (2016).
Read more (PubMed: 27388915) »