概述

  • 产品名称Anti-Claudin 5抗体
    参阅全部 Claudin 5 一抗
  • 描述
    兔多克隆抗体to Claudin 5
  • 经测试应用适用于: ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • 阳性对照
    • Lung

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab15106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 22547149
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
IHC-P 1/50.

靶标

  • 功能Plays a major role in tight junction-specific obliteration of the intercellular space.
  • 序列相似性Belongs to the claudin family.
  • 细胞定位Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Androgen withdrawal and apoptosis induced protein RVP1 like antibody
    • AWAL antibody
    • BEC 1 antibody
    • BEC1 antibody
    • Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody
    • Claudin-5 antibody
    • Claudin5 antibody
    • CLD5_HUMAN antibody
    • CLDN 5 antibody
    • Cldn5 antibody
    • CPETR L1 antibody
    • CPETRL 1 antibody
    • CPETRL1 antibody
    • TMDVCF antibody
    • TMVCF antibody
    • Transmembrane protein deleted in VCFS antibody
    • Transmembrane protein deleted in velocardiofacial syndrome antibody
    see all

Anti-Claudin 5 antibody 图像

  • ab15106 staining Claudin 5 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1.5% serum for 1 hour at 37°C; antigen retrieval was by heat mediation in a acetic acid. Samples were incubated with primary antibody (1/200 in blocking buffer) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab15106 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15106, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab15106 staining Claudin 5 in human lung by Immunohistochemistry (FFPE-sections).

  • ab15106 staining Claudin 5 in the Human HBMEC cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton in PBS and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-Claudin 5 antibody (ab15106) at 1/1000 dilution + Mouse lung tissue lysate at 30 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 25°C.

    Incubated for 15 hours at 4°C with the primary antibody.

    See Abreview

Anti-Claudin 5 antibody (ab15106)参考文献

This product has been referenced in:
  • Kudinov AE  et al. Musashi-2 (MSI2) supports TGF-ß signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis. Proc Natl Acad Sci U S A 113:6955-60 (2016). WB . Read more (PubMed: 27274057) »
  • Yamagami K  et al. Pirfenidone exhibits cardioprotective effects by regulating myocardial fibrosis and vascular permeability in pressure-overloaded hearts. Am J Physiol Heart Circ Physiol 309:H512-22 (2015). WB, IHC-P ; Mouse . Read more (PubMed: 26055790) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Mouse Cell (bEnd.3)
Permeabilization Yes - 0.1% Triton in PBS
Specification bEnd.3
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
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提交于 Nov 25 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HBMEC)
Permeabilization Yes - 0.1% Triton in PBS
Specification HBMEC
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
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提交于 Nov 25 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (15%)
Sample Mouse Tissue lysate - whole (lung)
Specification lung
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 08 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 37°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: acetic acid
Sample Mouse Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Paraformaldehyde
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提交于 Jul 30 2014

Thank you for your enquiry. Unfortunately, the immunogen sequence for ab15106 is proprietary and cannot be released. However, I can confirm that it is not in the extracellular loop (AA29-81) but is close to the C-terminal. This is also true for ab1...

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Thank you for your enquiry. The antibody originates outside of Abcam and the originator of the antibody does not wish to make the amino acid sequence available as it is considered commercially sensitive information. I can tell you, however, that th...

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Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you d...

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Thank you for your enquiry. Ab15106 has been tested for cross-reactivity with human (using lung tissue) but has not been tested yet for cross-reactivity with mouse. The immunogen used to generate this antibody was a synthetic peptide from the C ter...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"