Anti-Claudin 5抗体[1G4] (ab114027)

概述

  • 产品名称Anti-Claudin 5抗体[1G4]
    参阅全部 Claudin 5 一抗
  • 描述
    小鼠单克隆抗体[1G4] to Claudin 5
  • 经测试应用适用于: Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human Claudin 5 produced in HEK293T cells (NP_003268)

  • 阳性对照
    • ICC/IF: Transfected COS7 cells Flow Cyt: Transfected HEK293T cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Purified from mouse ascites fluids by affinity chromatography
  • 克隆单克隆
  • 克隆编号1G4
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab114027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

靶标

  • 功能Plays a major role in tight junction-specific obliteration of the intercellular space.
  • 序列相似性Belongs to the claudin family.
  • 细胞定位Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Androgen withdrawal and apoptosis induced protein RVP1 like antibody
    • AWAL antibody
    • BEC 1 antibody
    • BEC1 antibody
    • Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody
    • Claudin-5 antibody
    • Claudin5 antibody
    • CLD5_HUMAN antibody
    • CLDN 5 antibody
    • Cldn5 antibody
    • CPETR L1 antibody
    • CPETRL 1 antibody
    • CPETRL1 antibody
    • TMDVCF antibody
    • TMVCF antibody
    • Transmembrane protein deleted in VCFS antibody
    • Transmembrane protein deleted in velocardiofacial syndrome antibody
    see all

Anti-Claudin 5 antibody [1G4] 图像

  • HEK293T cells transfected with either pCMV6-ENTRY CLDN5 (Red) or empty vector control plasmid (Blue) were immunostained with ab114027 at 1/100 and then analyzed by flow cytometry
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CLDN5, using ab114027 at 1/100

Anti-Claudin 5 antibody [1G4] (ab114027)参考文献

ab114027 has not yet been referenced specifically in any publications.

Product Wall


The lab let me know that they routinely permeabilize when doing ICC or flow cytometry (see protocol below).


This antibody was made with the full-length protein and has not been epitope-mapped thus we do not know if it detects an extr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"