概述

  • 产品名称
    Anti-Claudin 4抗体
    参阅全部 Claudin 4 一抗
  • 描述
    兔多克隆抗体to Claudin 4
  • 经测试应用
    适用于: IHC-P, ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Pig
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • Extracts from HeLa cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA 1/5000.

靶标

  • 功能
    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • 疾病相关
    Note=CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • 序列相似性
    Belongs to the claudin family.
  • 细胞定位
    Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Claudin-4 antibody
    • Claudin4 antibody
    • CLD4_HUMAN antibody
    • CLDN 4 antibody
    • CLDN4 antibody
    • Clostridium perfringens enterotoxin receptor 1 antibody
    • Clostridium perfringens enterotoxin receptor antibody
    • CPE R antibody
    • CPE receptor antibody
    • CPE-R antibody
    • CPE-receptor antibody
    • CPER antibody
    • CPETR 1 antibody
    • CPETR antibody
    • CPETR1 antibody
    • hCPE R antibody
    • WBSCR8 antibody
    • Williams Beuren syndrome chromosome region 8 protein antibody
    • Williams-Beuren syndrome chromosomal region 8 protein antibody
    see all

Anti-Claudin 4 antibody 图像

  • All lanes : Anti-Claudin 4 antibody (ab53156) at 1/500 dilution

    Lane 1 : extracts from HeLa cells, untreated.
    Lane 2 : extracts from HeLa cells, treated with the immunizing peptide.


    Predicted band size : 22 kDa
    Observed band size : 22 kDa
  • Ab53156 staining Human colon. Staining is localised to the cell membrane.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab53156 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53156, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Claudin 4 antibody (ab53156)参考文献

This product has been referenced in:
  • Pasternak JA  et al. Claudin-4 undergoes age-dependent change in cellular localization on pig jejunal villous epithelial cells, independent of bacterial colonization. Mediators Inflamm 2015:263629 (2015). IHC . Read more (PubMed: 25948883) »
  • Hwang TL  et al. Claudin-4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase-2 and -9 expression. Oncol Lett 8:1367-1371 (2014). WB ; Human . Read more (PubMed: 25120725) »

See all 4 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Ileum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 6.0
Permeabilization
No
Specification
Ileum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Username

Alex Pasternak

Verified customer

提交于 Aug 19 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chimpanzee Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Colon
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 12 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (distal ileum)
Loading amount
40 µg
Specification
distal ileum
Gel Running Conditions
Reduced Denaturing (14)
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 30% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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