The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ICC/IF: Use at an assay dependent dilution (PMID 19129472).
IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Plays a major role in tight junction-specific obliteration of the intercellular space.
疾病相关Note=CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Formalin fixed paraffin embedded human tonsil stained with ab15104 (1:200, 10 min at room temperature).
Anti-Claudin 4 antibody (ab15104)参考文献
This product has been referenced in:
Lean QY et al. Orally Administered Enoxaparin Ameliorates Acute Colitis by Reducing Macrophage-Associated Inflammatory Responses. PLoS One10:e0134259 (2015).
Read more (PubMed: 26218284) »
Süren D et al. Loss of tight junction proteins (Claudin 1, 4, and 7) correlates with aggressive behavior in colorectal carcinoma. Med Sci Monit20:1255-62 (2014).
Read more (PubMed: 25038829) »