概述

  • 产品名称Anti-Claudin 4抗体
    参阅全部 Claudin 4 一抗
  • 描述
    兔多克隆抗体to Claudin 4
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Pig
  • 免疫原

    Synthetic peptide:

    CKPYSAKYSAARSAAASNYV

    , amino acids 191-209 of Human Claudin 4.

  • 阳性对照
    • Tested with tonsil and ovarian carcinoma cells.

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  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab15104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
IHC-P
  • 应用说明ICC/IF: Use at an assay dependent dilution (PMID 19129472).
    IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Plays a major role in tight junction-specific obliteration of the intercellular space.
    • 疾病相关Note=CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • 序列相似性Belongs to the claudin family.
    • 细胞定位Cell junction > tight junction. Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Claudin-4 antibody
      • Claudin4 antibody
      • CLD4_HUMAN antibody
      • CLDN 4 antibody
      • CLDN4 antibody
      • Clostridium perfringens enterotoxin receptor 1 antibody
      • Clostridium perfringens enterotoxin receptor antibody
      • CPE R antibody
      • CPE receptor antibody
      • CPE-R antibody
      • CPE-receptor antibody
      • CPER antibody
      • CPETR 1 antibody
      • CPETR antibody
      • CPETR1 antibody
      • hCPE R antibody
      • WBSCR8 antibody
      • Williams Beuren syndrome chromosome region 8 protein antibody
      • Williams-Beuren syndrome chromosomal region 8 protein antibody
      see all

    Anti-Claudin 4 antibody 图像

    • Anti-Claudin 4 antibody (ab15104) at 1 µg/ml + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 22 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 125 kDa,34 kDa,35 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds
    • Formalin fixed paraffin embedded human tonsil stained with ab15104 (1:200, 10 min at room temperature).

    Anti-Claudin 4 antibody (ab15104)参考文献

    This product has been referenced in:
    • Lean QY  et al. Orally Administered Enoxaparin Ameliorates Acute Colitis by Reducing Macrophage-Associated Inflammatory Responses. PLoS One 10:e0134259 (2015). IHC ; Mouse . Read more (PubMed: 26218284) »
    • Süren D  et al. Loss of tight junction proteins (Claudin 1, 4, and 7) correlates with aggressive behavior in colorectal carcinoma. Med Sci Monit 20:1255-62 (2014). IHC-P ; Human . Read more (PubMed: 25038829) »

    See all 9 Publications for this product

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Colon)
    Gel Running Conditions Reduced Denaturing (6-15% gradient gel)
    Loading amount 20 µg
    Specification Colon
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 28 2015

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Non-reduced Denaturing (15%)
    Sample Mouse Tissue lysate - whole (lung)
    Specification lung
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 30 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Adult Pancreas)
    Specification Adult Pancreas
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 20%
    Username

    Joby Westmoreland

    Verified customer

    提交于 Mar 10 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"