Anti-Claudin 3抗体(ab15102)
Key features and details
- Rabbit polyclonal to Claudin 3
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Chicken, Human, Pig
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Claudin 3抗体
参阅全部 Claudin 3 一抗 -
描述
兔多克隆抗体to Claudin 3 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Chicken, Human, Pig -
免疫原
Synthetic peptide within Mouse Claudin 3 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9Z0G9 -
常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15102于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (2) |
1/100.
|
IHC-P | (3) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
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ICC/IF
1/100. |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. -
疾病相关
Note=CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
序列相似性
Belongs to the claudin family. -
细胞定位
Cell junction > tight junction. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1365 Human
- Entrez Gene: 12739 Mouse
- Entrez Gene: 65130 Rat
- Omim: 602910 Human
- SwissProt: O15551 Human
- SwissProt: Q9Z0G9 Mouse
- SwissProt: Q63400 Rat
- Unigene: 647023 Human
see all -
别名
- C7orf1 antibody
- Claudin-3 antibody
- Claudin3 antibody
see all
图片
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ICC/IF image of ab15102 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15102, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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ab15102 (2µg/ml) staining Claudin 3 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong membrane staining of the mucosal epithelial cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (68)
ab15102 被引用在 68 文献中.
- Cuccato M et al. Assessment of Antimicrobial Effects on Broiler Gut Barrier Through Histopathology and Immunohistochemistry of Tight-Junction Proteins. Front Vet Sci 9:830073 (2022). PubMed: 35425830
- Iwaniak P et al. Dietary Alpha-Ketoglutarate Partially Abolishes Adverse Changes in the Small Intestine after Gastric Bypass Surgery in a Rat Model. Nutrients 14:N/A (2022). PubMed: 35631203
- Wu J et al. Limosilactobacillus reuteri SLZX19-12 Protects the Colon from Infection by Enhancing Stability of the Gut Microbiota and Barrier Integrity and Reducing Inflammation. Microbiol Spectr 10:e0212421 (2022). PubMed: 35658572
- Yin Y et al. Protective Effect of Prim-O-Glucosylcimifugin on Ulcerative Colitis and Its Mechanism. Front Pharmacol 13:882924 (2022). PubMed: 35662727
- Kim WS et al. High Expression of Claudin-4 Is Associated with Synchronous Tumors in Patients with Early Gastric Cancer. J Clin Med 11:N/A (2022). PubMed: 35743616