概述

  • 产品名称Anti-Claudin 3抗体
    参阅全部 Claudin 3 一抗
  • 描述
    兔多克隆抗体to Claudin 3
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Claudin 3.

  • 阳性对照
    • This antibody gave a positive signal in both Human Ovary and Human Colon tissue lysates as well as the following whole cell lysates: Caco2; SW480; LoVo. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal prostate.

性能

应用

Our Abpromise guarantee covers the use of ab116165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 23 kDa).
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
  • 疾病相关Note=CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • 序列相似性Belongs to the claudin family.
  • 细胞定位Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C7orf1 antibody
    • Claudin-3 antibody
    • Claudin3 antibody
    • CLD3_HUMAN antibody
    • CLDN 3 antibody
    • Cldn3 antibody
    • Clostridium perfringens enterotoxin receptor 2 antibody
    • CPE R2 antibody
    • CPE receptor 2 antibody
    • CPE-R 2 antibody
    • CPE-receptor 2 antibody
    • CPETR 2 antibody
    • CPETR2 antibody
    • HRVP 1 antibody
    • HRVP1 antibody
    • Rat ventral prostate 1 like protein antibody
    • Rat ventral prostate.1 protein homolog antibody
    • RVP1 antibody
    • Ventral prostate.1 like protein antibody
    • Ventral prostate.1 protein homolog antibody
    see all

Anti-Claudin 3 antibody 图像

  • All lanes : Anti-Claudin 3 antibody (ab116165) at 1 µg/ml

    Lane 1 : Human ovary tissue lysate - total protein (ab30222)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : Human ovary tissue lysate - total protein (ab30222) with Immunizing peptide at 1 µg/ml
    Lane 7 : Human colon tissue lysate - total protein (ab30051) with Immunizing peptide at 1 µg/ml
    Lane 8 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 9 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 10 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • IHC image of Claudin 3 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116165, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Claudin 3 antibody (ab116165)参考文献

ab116165 has not yet been referenced specifically in any publications.

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