概述

  • 产品名称
    Anti-Claudin 23抗体
    参阅全部 Claudin 23 一抗
  • 描述
    兔多克隆抗体to Claudin 23
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Pig
  • 免疫原

    Synthetic peptide derived from a region between residues 96-150 of Human Claudin 23.

  • 阳性对照
    • HepG2 cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.2 - 0.5 µg/ml. Predicted molecular weight: 32 kDa.

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

IHC-P Use at an assay dependent concentration.

靶标

Anti-Claudin 23 antibody 图像

  • Anti-Claudin 23 antibody (ab23355) at 1 µg/ml + HepG2 cell lysate

    Predicted band size : 32 kDa
  • All lanes : Anti-Claudin 23 antibody (ab23355) at 1 µg/ml

    Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 32 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa,70 kDa,80 kDa. We are unsure as to the identity of these extra bands.
  • ab23355 staining paraffin embedded human placenta tissue at 5 ug/ml.
  • ab23355 staining Claudin 23 in kidney cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with methanol, permeabilized with Triton X-100 and blocked with 5% BSA for 30 minutes. Samples were incubated with primary antibody (1/600 in PBS + 0.5% BSA + 0.1% Triton X-100) for 1 hour at 20°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/800) was used as the secondary antibody.

    See Abreview

Anti-Claudin 23 antibody (ab23355)参考文献

This product has been referenced in:
  • Maryan N  et al. Regulation of the expression of claudin 23 by the enhancer of zeste 2 polycomb group protein in colorectal cancer. Mol Med Rep 12:728-36 (2015). Read more (PubMed: 25695204) »
  • Gareus R  et al. Normal epidermal differentiation but impaired skin-barrier formation upon keratinocyte-restricted IKK1 ablation. Nat Cell Biol 9:461-9 (2007). Read more (PubMed: 17351639) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (LUNG)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
30 µg
Specification
LUNG
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jan 27 2016

According to uniprot http://www.uniprot.org/uniprot/Q96B33, human Claudin 23 is a 32 kDa protein. There are not any other isoforms or cleavage fragments reported on uniprot, but Claudin 23 is not extensively studies, so there may be signal or propeptid...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse kidney tissue)
Permeabilization
No
Specification
Mouse kidney tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Lei Pei

Verified customer

提交于 May 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney)
Specification
kidney
Fixative
Methanol
Permeabilization
Yes - Triton X
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Sep 05 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E14.5 wildtype epidermis)
Specification
E14.5 wildtype epidermis
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 19 2012

Merci pour votre requête.
La méthode de démasquage est une étape très importante à optimiser. Nous conseillons donc en générale d'optimiser cette étape dans chaque laboratoire.
Effectivement, si un anticorps avait fonctionné bien en HIER avec...

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Merci de nous avoir contactés.
Pour le démasquage antigène nous recommandons la méthode HIER avec du tampon Tris-EDTA pH 9.0. Comme le montre une publication de 2005 (Emoto et al., J Histochem Cytochem. 2005 Nov; 53 (11) : 1311-21, tableau nº2) le...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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