概述

  • 产品名称
    Anti-Claudin 17抗体
    参阅全部 Claudin 17 一抗
  • 描述
    兔多克隆抗体to Claudin 17
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: GSNERAKAYL LGTSGVLFIL TGIFVLIPVS WTANIIIRDF YNPAIHIGQK, corresponding to internal sequence amino acids 109-158 of Human Claudin 17

  • 阳性对照
    • Jurkat cell lysate for western blot and human lung and spleen for immunohistochemistry.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: None.
    Constituents: 2% Sucrose, PBS.
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    This antibody was purified by chromatography. 
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性
    Claudin proteins are a family of transmembrane proteins associated with tight junctions, and different tissues exhibit different Claudin composition. There are 18 homolgs present in humans, and mutations in any of these proteins cause a variety of human disease, this indicates their physiological importance. CLDN17, clustered with CLDN8 at human chromosome 21q22.11, is a four transmembrane protein with WWCC motif, defined by W-X(17-22)-W-X(2)-C-X(8-10)-C.
  • 细胞定位
    Integral membrane protein. Tight junctions.
  • 数据库链接
  • 别名
    • CLDN17 antibody
    • Human CLDN17 gene for claudin 17 antibody

Anti-Claudin 17 antibody 图像

  • Anti-Claudin 17 antibody (ab23333) at 2 µg/ml + Jurkat cell lysate (10ug)

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution.

    Predicted band size : 25 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Paraffin-embedded sections of Human spleen cells incubated with ab23333. The antibody was used at a range of 4.0-8.0µg/ml. Cells stained positive for Cluadin 17 are indicated by arrows. 400x magnification.

  • Paraffin-embedded sections of Human alveolar cells incubated with ab23333. The antibody was used at a range of 4.0-8.0µg/ml. Cells stained positive for Claudin 17 are indicated by arrows. 400x magnification.

Anti-Claudin 17 antibody (ab23333)参考文献

This product has been referenced in:
  • Gao M  et al. The distinct expression patterns of claudin-10, -14, -17 and E-cadherin between adjacent non-neoplastic tissues and gastric cancer tissues. Diagn Pathol 8:205 (2013). Human . Read more (PubMed: 24325792) »
  • Farwick M  et al. Fifty-kDa hyaluronic acid upregulates some epidermal genes without changing TNF-a expression in reconstituted epidermis. Skin Pharmacol Physiol 24:210-7 (2011). WB ; Human . Read more (PubMed: 21412035) »

See all 3 Publications for this product

Product Wall

Thank you for your reply.
I am sorry to hear that the tips did not help to improve the results.
Since the antibody is being used in an untested application (ICC/IF), our Abpromise guarantee does not apply (thus I cannot refund the antibody). H...

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Thank you for submitting an Abreview for ab23333. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested application, we would like to follow up on this to see ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney)
Specification
kidney
Fixative
Methanol
Permeabilization
Yes - Triton X
Blocking step
BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 12 2012

Thank you for getting back to me. Generally if an immunogen forms an alignment of 6 or more consecutive residues I would consider it possible that the antibody would react against the homolog of the species. However, if it is a polyclonal antisera ...

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Thank you for your enquiry. I have performed a sequence analysis of your fish sequence with all of immunogens used to raise our antibodies against Claudin. Unfortunately none had significant sequence similarity that would make me consider reactivit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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