概述

  • 产品名称
    Anti-Claudin 10抗体
    参阅全部 Claudin 10 一抗
  • 描述
    兔多克隆抗体to Claudin 10
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • Extracts from LOVO cells.

性能

应用

Our Abpromise guarantee covers the use of ab52234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/300 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IHC-P Use at an assay dependent concentration.

靶标

图片

  • All lanes : Anti-Claudin 10 antibody (ab52234) at 1/300 dilution

    Lane 1 : Extracts from LOVO cells, untreated.
    Lane 2 : Extracts from LOVO cells, treated with the immunising peptide.


    Predicted band size : 24 kDa
    Observed band size : 24 kDa
  • ab52234 staining Claudin 10 in sections of Human proximal tubular cells by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in methanol prior to blocking with 5% BSA for 30 minutess at 20°C. The primary antibody was diluted 1/300 in 0.5% BSA/0.1% Triton X-100 and incubated with the sample for 1 hour at 20°C. An Alexa Fluor® 488-conjugated Donkey anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/800.

    See Abreview

文献

This product has been referenced in:
  • Tang J  et al. Effects of mesenchymal stem cells on interleukin-1ß-treated chondrocytes and cartilage in a rat osteoarthritic model. Mol Med Rep 12:1753-60 (2015). WB . Read more (PubMed: 25892273) »
  • Aschauer L  et al. Delineation of the key aspects in the regulation of epithelial monolayer formation. Mol Cell Biol 33:2535-50 (2013). Read more (PubMed: 23608536) »

See all 2 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Proximal tubular cells)
Loading amount
30 µg
Specification
Proximal tubular cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 May 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Proximal tubular cells)
Specification
Proximal tubular cells
Fixative
Methanol
Antigen retrieval step
None
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 May 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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