Anti-Claudin 1抗体- Loop 1 (ab63070)

概述

  • 产品名称Anti-Claudin 1抗体- Loop 1
    参阅全部 Claudin 1 一抗
  • 描述
    兔多克隆抗体to Claudin 1 - Loop 1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the Loop 1 region of human Claudin 1.

  • 阳性对照
    • Jurkat cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明ab63070 was purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab63070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

靶标

  • 功能Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
    (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).
  • 组织特异性Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
  • 疾病相关Ichthyosis-sclerosing cholangitis neonatal syndrome
  • 序列相似性Belongs to the claudin family.
  • 细胞定位Cell junction, tight junction. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Claudin-1 antibody
    • Claudin1 antibody
    • CLD 1 antibody
    • CLD1 antibody
    • CLD1_HUMAN antibody
    • CLDN 1 antibody
    • Cldn1 antibody
    • ILVASC antibody
    • SEMP 1 antibody
    • SEMP1 antibody
    • Senescence associated epithelial membrane protein 1 antibody
    • Senescence associated epithelial membrane protein antibody
    • Senescence-associated epithelial membrane protein antibody
    see all

Anti-Claudin 1 antibody - Loop 1 图像

  • Anti-Claudin 1 antibody - Loop 1 (ab63070) at 1/60 dilution + Jurkat cell lysates at 35 µg

    Predicted band size : 23 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hCLDN1-Loop1 (ab63070), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Anti-Claudin 1 antibody - Loop 1 (ab63070)参考文献

This product has been referenced in:
  • Barretto N  et al. Determining the involvement and therapeutic implications of host cellular factors in hepatitis C virus cell-to-cell spread. J Virol 88:5050-61 (2014). Read more (PubMed: 24554660) »
  • Martin DN & Uprichard SL Identification of transferrin receptor 1 as a hepatitis C virus entry factor. Proc Natl Acad Sci U S A 110:10777-82 (2013). Read more (PubMed: 23754414) »

See all 3 Publications for this product

Product Wall

Vielen Dank für Ihren Anruf. Bei dem Immunogen für den anti-Claudin 1- Antikörper ab63070 handelt es sich um ein Peptid, das zwischen den Aminosäuren 54 und 83 des humanen Claudins 1 (und damit im ersten extrazellulären loop) lokalisiert ist (CVSQST...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"