概述

  • 产品名称
    Anti-Claudin 1抗体
    参阅全部 Claudin 1 一抗
  • 描述
    小鼠单克隆抗体to Claudin 1
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-212 of Human Claudin 1

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab211737 as a replacement.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 同种型
    IgG2a
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab56417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 23 kDa.
IHC-P Use a concentration of 3 µg/ml.

靶标

  • 功能
    Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
    (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).
  • 组织特异性
    Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
  • 疾病相关
    Ichthyosis-sclerosing cholangitis neonatal syndrome
  • 序列相似性
    Belongs to the claudin family.
  • 细胞定位
    Cell junction, tight junction. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Claudin-1 antibody
    • Claudin1 antibody
    • CLD 1 antibody
    • CLD1 antibody
    • CLD1_HUMAN antibody
    • CLDN 1 antibody
    • Cldn1 antibody
    • ILVASC antibody
    • SEMP 1 antibody
    • SEMP1 antibody
    • Senescence associated epithelial membrane protein 1 antibody
    • Senescence associated epithelial membrane protein antibody
    • Senescence-associated epithelial membrane protein antibody
    see all

图片

  • Claudin 1 antibody (ab56417) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.


  • Predicted band size : 23 kDa
    Claudin 1 antibody (ab56417) at 1ug/lane + PRC/PRF/5 cell lysate at 25ug/lane.

文献

This product has been referenced in:
  • Takahashi S  et al. Expression of adhesion molecules and epithelial-mesenchymal transition factors in medullary carcinoma of the colorectum. Hum Pathol 46:1257-66 (2015). IHC-P ; Human . Read more (PubMed: 26208847) »
  • Tokuhara Y  et al. Claudin-1, but not claudin-4, exhibits differential expression patterns between well- to moderately-differentiated and poorly-differentiated gastric adenocarcinoma. Oncol Lett 10:93-98 (2015). Read more (PubMed: 26170982) »

See all 5 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse pancreas)
Specification
mouse pancreas
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 16°C
Username

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Verified customer

提交于 Jul 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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