Anti-Clathrin light chain抗体[CON.1] (ab24579)

概述

  • 产品名称
    Anti-Clathrin light chain抗体[CON.1]
    参阅全部 Clathrin light chain 一抗
  • 描述
    小鼠单克隆抗体[CON.1] to Clathrin light chain
  • 特异性
    ab24579 recognises Clathrin Light Chain.
  • 经测试应用
    适用于: IHC-P, WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Mammal
  • 免疫原

    Synthetic peptide:

    EEDPAAAFLAQQESEIAGIEND

    , corresponding to amino acids 23/44 of Cow Clathrin light chain

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

应用

Our Abpromise guarantee covers the use of ab24579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000. (see Abreview).
WB 1/250. Predicted molecular weight: 25-29 kDa.
ICC/IF Use at an assay dependent concentration.
IP 1/200.

靶标

图片

  • Formalin fixed HeLa cells double stained with the monoclonal antibody to clathrin, (ab24579, Red) and giantin (green). The nucleus was visualized with DAPI stain.
  • ab74579 staining Clatherin light chain in human prostate carcinoma tissue by immunohistochemistry (IHC-P - formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 1 hour at 21 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were incubated with primary antibody 1/1000 (PBS) for 1 hour at 21 °C. A Biotin-conjugated Donkey polyclonal to mouse IgG, dilution 1/200, was used as secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Poulsen ET  et al. New Insights to Clathrin and Adaptor Protein 2 for the Design and Development of Therapeutic Strategies. Int J Mol Sci 16:29446-53 (2015). IP . Read more (PubMed: 26690411) »
  • Martins RP  et al. Pyroptosis and adaptive immunity mechanisms are promptly engendered in mesenteric lymph-nodes during pig infections with Salmonella enterica serovar Typhimurium. Vet Res 44:120 (2013). WB . Read more (PubMed: 24308825) »

See all 4 Publications for this product

客户评价及客户问答

Thank you for contacting Abcam.

Unfortunately we do not have any western blot images that I can send you, the 1/250 dilution is the recommendation that we received from our sourcing lab.

Although it does seem high, I would recommend...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate Carcinoma)
Specification
Prostate Carcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Aug 19 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (neuronal stem cells)
Specification
neuronal stem cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,5% Saponin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. Dominik Schelshorn

Verified customer

提交于 Feb 05 2008

Thank you for your enquiry and for completing our technical questionnaire. I am sorry to hear that you have been having difficulties with this antibody. I have read your questionnaire and I have a few comments. Please can you tell me the molecu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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