山羊多克隆抗体to Clathrin light chain
Expected to recognize all reported isoforms of light chains a and b.
Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Macaque monkey
from Human Clathrin light chain(NP_001824.1; NP_009027.1; NP_001070145.1; NP_001825.1; NP_009028.1)
Human frontal cortex lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/32000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 23 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
Cytoplasmic vesicle membrane. Membrane > coated pit. Cytoplasmic face of coated pits and vesicles.
Information by UniProt
Clathrin light chain A antibody
Clathrin light chain B antibody
Clathrin light chain LCA antibody
Western blot - Clathrin light chain antibody (ab77542)
Anti-Clathrin light chain antibody (ab77542) at 0.1 µg/ml + Human frontal cortex lysate in RIPA buffer at 35 µg
Predicted band size: 23 kDa Observed band size: 23 kDa Additional bands at: 35 kDa (possible isoform) The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"