Anti-Clathrin heavy chain抗体[TD.1] (ab24578)
Key features and details
- Mouse monoclonal [TD.1] to Clathrin heavy chain
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Clathrin heavy chain抗体[TD.1]
参阅全部 Clathrin heavy chain 一抗 -
描述
小鼠单克隆抗体[TD.1] to Clathrin heavy chain -
宿主
Mouse -
特异性
This antibody reacts with clathrin heavy chains (CHC). -
经测试应用
适用于: ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
This antibody was raised using the globular N terminal domain of clathrin heavy chain.
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阳性对照
- Methanol fixed HeLa cells for IF.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.03% Thimerosal (merthiolate) -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
TD.1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab24578于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. -
序列相似性
Belongs to the clathrin heavy chain family. -
细胞定位
Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 1213 Human
- Omim: 118955 Human
- SwissProt: P53675 Human
- SwissProt: Q00610 Human
- Unigene: 491351 Human
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别名
- CHC17 antibody
- Clathrin heavy chain antibody
- Clathrin heavy chain 1 antibody
see all
图片
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ab24578 at a 1/200 dilution staining clathrin triskelion (blue) throughout the methanol fixed HeLa cells by Immunocytochemistry/Immunofluorescence. The Golgi is brilliantly stained by Giantin (yellow). Secondary antibodies used were a goat anti-mouse alexafluor AF546 and a goat anti-rabbit alexafluor AF488 applied at 1 ug/mL. The DNA was visualized with DAPI at 1 ug/mL. The colors of this image were altered.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab24578 被引用在 9 文献中.
- Mukenhirn M et al. Role of Clathrin Light Chains in Regulating Invadopodia Formation. Cells 10:N/A (2021). PubMed: 33672612
- Pechstein A et al. Vesicle Clustering in a Living Synapse Depends on a Synapsin Region that Mediates Phase Separation. Cell Rep 30:2594-2602.e3 (2020). PubMed: 32101738
- Vassilopoulos S et al. Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization. J Cell Biol 205:377-93 (2014). PubMed: 24798732
- Chowdhury D et al. Tyrosine phosphorylation regulates the endocytosis and surface expression of GluN3A-containing NMDA receptors. J Neurosci 33:4151-64 (2013). PubMed: 23447623
- Kitahara H et al. COP35, a cholangiocarcinoma-binding oligopeptide, interacts with the clathrin heavy chain accompanied by GRP78. Mol Cancer Res 9:688-701 (2011). IHC-P ; Human . PubMed: 21571834
- Harel A et al. Evidence for CALM in directing VAMP2 trafficking. Traffic 9:417-29 (2008). PubMed: 18182011
- Praznikar ZJ et al. A presynaptically toxic secreted phospholipase A2 is internalized into motoneuron-like cells where it is rapidly translocated into the cytosol. Biochim Biophys Acta 1783:1129-39 (2008). PubMed: 18261469
- Bushlin I et al. Clathrin assembly protein AP180 and CALM differentially control axogenesis and dendrite outgrowth in embryonic hippocampal neurons. J Neurosci 28:10257-71 (2008). PubMed: 18842885
- Borlido J et al. Clathrin is spindle-associated but not essential for mitosis. PLoS ONE 3:e3115 (2008). WB ; Human . PubMed: 18769625