概述

  • 产品名称
    Anti-Claspin抗体
    参阅全部 Claspin 一抗
  • 描述
    兔多克隆抗体to Claspin
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1126-1330 of Human Claspin (NM_022111).

  • 阳性对照
    • 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab94945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 150 kDa.

靶标

  • 功能
    Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
  • 序列相似性
    Belongs to the claspin family.
  • 结构域
    The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.
  • 翻译后修饰
    Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Claspin antibody
    • claspin homolog (Xenopus laevis) antibody
    • Claspin homolog antibody
    • CLSPN antibody
    • CLSPN protein antibody
    • CLSPN_HUMAN antibody
    • hClaspin antibody
    • Hu Claspin antibody
    see all

图片

  • Anti-Claspin antibody (ab94945) at 1/500 dilution + 293T cell lysate

    Predicted band size : 150 kDa

实验方案

文献

ab94945 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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