概述

  • 产品名称Anti-CLASP2抗体
    参阅全部 CLASP2 一抗
  • 描述
    兔多克隆抗体to CLASP2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the Y1019 region of Human CLASP2 (UniProt ID O75122).

  • 阳性对照
    • Mouse spleen tissue lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab95005 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG

应用

Our Abpromise guarantee covers the use of ab95005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 141 kDa.

靶标

  • 功能Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed:26003921). Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed:16824950). This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed:16866869, PubMed:16914514). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.
  • 组织特异性Brain-specific.
  • 序列相似性Belongs to the CLASP family.
    Contains 8 HEAT repeats.
  • 结构域The two SXIP sequence motifs mediate interaction with MAPRE1; this is necessary for targeting to growing microtubule plus ends.
    Two TOG regions display structural characteristics similar to HEAT repeat domains and mediate interaction with microtubules.
  • 翻译后修饰Phosphorylated by GSK3B. Phosphorylation reduces MAPRE1 binding (PubMed:26003921). Phosphorylation by GSK3B may negatively regulate binding to microtubule lattices in lamella.
  • 细胞定位Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Golgi apparatus. Golgi apparatus, trans-Golgi network. Cell membrane. Cell projection, ruffle membrane. Localizes to microtubule plus ends (PubMed:15631994). Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase (PubMed:16866869, PubMed:16914514). In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2 (PubMed:16824950). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CLAP2_HUMAN antibody
    • CLASP 2 antibody
    • Clasp2 antibody
    • CLIP associating protein 2 antibody
    • CLIP-associating protein 2 antibody
    • Cytoplasmic linker associated protein 2 antibody
    • Cytoplasmic linker-associated protein 2 antibody
    • hOrbit2 antibody
    • KIAA0627 antibody
    • Multiple asters (Mast) like homolog 2 antibody
    • Protein Orbit homolog 2 antibody
    see all

Anti-CLASP2 antibody 图像

  • Anti-CLASP2 antibody (ab95005) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg

    Predicted band size : 141 kDa

Anti-CLASP2 antibody (ab95005)参考文献

ab95005 has not yet been referenced specifically in any publications.

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