Anti-CLASP1抗体[KT67] (ab95372)
Key features and details
- Rat monoclonal [KT67] to CLASP1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-CLASP1抗体[KT67]
参阅全部 CLASP1 一抗 -
描述
大鼠单克隆抗体[KT67] to CLASP1 -
宿主
Rat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Fusion protein corresponding to Human CLASP1 (N terminal).
Database link: Q7Z460 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
KT67 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95372于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/5000. Predicted molecular weight: 169 kDa.
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说明 |
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WB
1/500 - 1/5000. Predicted molecular weight: 169 kDa. |
靶标
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功能
Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. -
序列相似性
Belongs to the CLASP family.
Contains 7 HEAT repeats. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Golgi apparatus > trans-Golgi network. Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2. - Information by UniProt
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数据库链接
- Entrez Gene: 23332 Human
- Omim: 605852 Human
- SwissProt: Q7Z460 Human
- Unigene: 469840 Human
- Unigene: 708183 Human
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别名
- 1700030C23Rik antibody
- 5730583A19Rik antibody
- B130045P17Rik antibody
see all
图片
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All lanes : Anti-CLASP1 antibody [KT67] (ab95372) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CLASP1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 169 kDaLanes 1 - 4: Merged signal (red and green). Green - ab95372 observed at 169 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab95372 was shown to specifically react with CLASP1 in wild-type HAP1 cells as signal was lost in CLASP1 knockout cells. Wild-type and CLASP1 knockout samples were subjected to SDS-PAGE. Ab95372 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab95372 尚未被引用在任何文献中。