概述

  • 产品名称Anti-CLASP1抗体
    参阅全部 CLASP1 一抗
  • 描述
    兔多克隆抗体to CLASP1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1488 and 1538 of Human CLASP1 (NP_056097.1)

  • 阳性对照
    • HeLa whole cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab85919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 169 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.
  • 序列相似性Belongs to the CLASP family.
    Contains 7 HEAT repeats.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Golgi apparatus > trans-Golgi network. Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1700030C23Rik antibody
    • 5730583A19Rik antibody
    • B130045P17Rik antibody
    • CLAP1_HUMAN antibody
    • clasp1 antibody
    • CLIP associating protein 1 antibody
    • CLIP associating protein CLASP1 antibody
    • CLIP-associating protein 1 antibody
    • Cytoplasmic linker associated protein 1 antibody
    • Cytoplasmic linker-associated protein 1 antibody
    • DKFZp686D1968 antibody
    • DKFZp686H2039 antibody
    • FLJ33821 antibody
    • FLJ41222 antibody
    • hOrbit1 antibody
    • KIAA0622 antibody
    • MAST1 antibody
    • MGC131895 antibody
    • mKIAA0622 antibody
    • Multiple asters 1 antibody
    • Multiple asters homolog 1 antibody
    • Protein Orbit homolog 1 antibody
    see all

Anti-CLASP1 antibody 图像

  • All lanes : Anti-CLASP1 antibody (ab85919) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 169 kDa
    Observed band size : 171 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 115 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of Human CLASP1 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85919 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-CLASP1 antibody (ab85919)参考文献

ab85919 has not yet been referenced specifically in any publications.

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