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Immunogen was a synthetic peptide, which represented a portion of human Casein kinase 2, alpha 2 polypeptide encoded within exon 11 (LocusLink ID 1459).
CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis.
Our Abpromise guarantee covers the use of ab10474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 4-10 µg/mg of lysate.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).|
Detection of human CKII alpha prime polypeptide by Western blot and immunoprecipitation.
A. RIPA extract (30 mg) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v).
B. RIPA extract (30 mg) from HeLa cells treated with CKII alpha, CKII alpha prime, or vimentin (v) siRNA.
C. RIPA extract (0.5mg) from HeLa cells.
Antibody: ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 mg/0.5 mg lysate (C) for IP.
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