Anti-CKII alpha (phospho T360 + S362)抗体(ab119410)

概述

  • 产品名称Anti-CKII alpha (phospho T360 + S362)抗体
    参阅全部 CKII alpha 一抗
  • 描述
    兔多克隆抗体to CKII alpha (phospho T360 + S362)
  • 特异性ab119410 detects endogenous levels of Human CKII alpha only when phosphorylated at Threm=onine 360/Srine 362
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, surrounding amino acids (VTPpPSpPL) phosphorylated at threonine 360 and Serine 362 of Human CKII alpha (NP_001894.2).

  • 阳性对照
    • Recombinant proteins CKII alpha wt, CKII alpha T360A, CKII alpha S362A, CKII alphaT360/S362A phoshorylated by ERK2

性能

应用

Our Abpromise guarantee covers the use of ab119410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).

靶标

  • 功能Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Casein kinase 2 alpha 1 polypeptide antibody
    • Casein kinase II alpha 1 antibody
    • Casein kinase II alpha 1 subunit antibody
    • Casein kinase II alpha subunit antibody
    • Casein kinase II subunit alpha antibody
    • CK II alpha antibody
    • CK II antibody
    • CK2 alpha antibody
    • CK2 catalytic subunit alpha antibody
    • CK2A1 antibody
    • CKII antibody
    • CKIIalpha antibody
    • CSK21_HUMAN antibody
    • CSNK2A1 antibody
    see all

Anti-CKII alpha (phospho T360 + S362) antibody 图像

  • All lanes : Anti-CKII alpha (phospho T360 + S362) antibody (ab119410) at 1/1000 dilution

    Lane 1 : CKII alpha
    Lane 2 : CKII alpha + ERK2
    Lane 3 : CKII alpha T360A
    Lane 4 : CKII alpha T360A + ERK2
    Lane 5 : CKII alpha S362A
    Lane 6 : CKII alpha S362A + ERK2
    Lane 7 : CKII alpha T360/S362A
    Lane 8 : CKII alpha T360/S362A + ERK2


    Predicted band size : 45 kDa
  • Anti-CKII alpha (phospho T360 + S362) antibody (ab119410) at 1/1000 dilution + C57BL/6 Mouse splenocyte whole cell lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-rabbit monoclonal IgG at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Tomar Ghansah

    See Abreview

Anti-CKII alpha (phospho T360 + S362) antibody (ab119410)参考文献

ab119410 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (C57BL/6 Mouse splenocytes)
Loading amount 40 µg
Specification C57BL/6 Mouse splenocytes
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Tomar Ghansah

Verified customer

提交于 Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"