Anti-CKII alpha抗体(ab10468)


  • 产品名称Anti-CKII alpha抗体
    参阅全部 CKII alpha 一抗
  • 描述
    兔多克隆抗体to CKII alpha
  • 特异性Reacts with alpha subunit.
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Immunogen was a synthetic peptide representing a portion of human Casein kinase 2, alpha 1 polypeptide which lies somewhere between aa300 and aa350.

  • 阳性对照
    • RIPA extract from HeLa cells treated with CKII alpha siRNA or vimentin siRNA.



Our Abpromise guarantee covers the use of ab10468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/3000 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/250 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 5 µg/ml.


Anti-CKII alpha antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling CKII alpha with ab10468 at 1/1000 (1µg/ml). Detection: DAB.
  • ICC/IF image of ab10468 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10468, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • developed using the ECL technique

    Predicted band size : 45 kDa

    Exposure time : 1 minute

    Western blot analysis of RIPA extract (30 µg) from HeLa cells treated with CKII alpha siRNA or vimentin siRNA (v). ab10468 was used at 0.33 or 0.1 µg/ml.


Anti-CKII alpha antibody (ab10468)参考文献

ab10468 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and patience. Under the conditions tested, which included WB detection at optimized dilutions using lysate from cells expressing endogenous amounts of the subunits, they were specific as specified. That is, ab10466, ab10468 a...

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Thank you for your enquiry. The immunogen information for the products are as follows: Ab10466- The immunogen used lies between aa 1-50. Ab10468- The immunogen used lies between aa 300-350. Ab10470- The immunogen used lies between aa350-391. ...

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