Anti-CSNK2A1抗体(ab10466)
Key features and details
- Rabbit polyclonal to CSNK2A1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CSNK2A1抗体
参阅全部 CSNK2A1 一抗 -
描述
兔多克隆抗体to CSNK2A1 -
宿主
Rabbit -
特异性
Reacts with alpha subunit. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
免疫原
Synthetic peptide corresponding to Human CSNK2A1 aa 1-100.
Database link: 1457 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antibodies were affinity purified using the peptide immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10466于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).
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说明 |
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WB
1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa). |
靶标
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功能
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 432370 Chicken
- Entrez Gene: 282419 Cow
- Entrez Gene: 1457 Human
- Entrez Gene: 12995 Mouse
- Entrez Gene: 116549 Rat
- Entrez Gene: 399319 Xenopus laevis
- Omim: 115440 Human
- SwissProt: P21868 Chicken
see all -
别名
- Casein kinase 2 alpha 1 polypeptide antibody
- Casein kinase 2 alpha antibody
- Casein kinase II alpha 1 antibody
see all
图片
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All lanes : Anti-CSNK2A1 antibody (ab10466) at 1/2500 dilution
Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 45 kDa -
All lanes : Anti-CSNK2A1 antibody (ab10466) at 1/5000 dilution (LI-COR Blocker (1:1) in PBS + 0.1% Tween for 3 hours)
Lane 1 : Whole cell lysate of HeLa cells
Lane 2 : siRNA to CK2 alpha in HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : An Alexa Fluor®680-conjugated Goat anti-rabit polyclonal at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Blocking Step: 50% LI-COR® Odyssey® Blocking Buffer for 45 minutes at room temperature.
All non-specific bands were very weak.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab10466 被引用在 11 文献中.
- Caefer DM et al. The Okur-Chung Neurodevelopmental Syndrome Mutation CK2K198R Leads to a Rewiring of Kinase Specificity. Front Mol Biosci 9:850661 (2022). PubMed: 35517865
- Raju P et al. Inactivation of paracellular cation-selective claudin-2 channels attenuates immune-mediated experimental colitis in mice. J Clin Invest 130:5197-5208 (2020). PubMed: 32516134
- Mohl BP & Roy P Hsp90 Chaperones Bluetongue Virus Proteins and Prevents Proteasomal Degradation. J Virol 93:N/A (2019). PubMed: 31375577
- Mohl BP & Roy P Cellular Casein Kinase 2 and Protein Phosphatase 2A Modulate Replication Site Assembly of Bluetongue Virus. J Biol Chem 291:14566-74 (2016). WB ; Human . PubMed: 27226558
- Herhaus L et al. Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization. Sci Signal 8:ra35 (2015). PubMed: 25872870