概述

  • 产品名称
    Anti-CKII alpha抗体
    参阅全部 CKII alpha 一抗
  • 描述
    兔多克隆抗体to CKII alpha
  • 特异性
    Reacts with alpha subunit.
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    The immunogen was a synthetic peptide, which represented a portion of human casein kinase 2, alpha 1 polypeptide encoded within exon 3 (LocusLink ID 1457).

  • 阳性对照
    • RIPA extract from HeLa cells treated with CKII alpha siRNA or vimentin siRNA.

性能

应用

Our Abpromise guarantee covers the use of ab10466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

图片

  • All lanes : Anti-CKII alpha antibody (ab10466) at 1/2500 dilution

    Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 45 kDa
  • All lanes : Anti-CKII alpha antibody (ab10466) at 1/5000 dilution (LI-COR Blocker (1:1) in PBS + 0.1% Tween for 3 hours)

    Lane 1 : Whole cell lysate of HeLa cells
    Lane 2 : siRNA to CK2 alpha in HeLa cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    An Alexa Fluor®680-conjugated Goat anti-rabit polyclonal at 1/10000 dilution

    Predicted band size : 45 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Svetlana Khoronenkova

    See Abreview

  • ICC/IF image of ab10466 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10466, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Mohl BP & Roy P Cellular Casein Kinase 2 and Protein Phosphatase 2A Modulate Replication Site Assembly of Bluetongue Virus. J Biol Chem 291:14566-74 (2016). WB ; Human . Read more (PubMed: 27226558) »
  • Herhaus L  et al. Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization. Sci Signal 8:ra35 (2015). Read more (PubMed: 25872870) »

See all 8 Publications for this product

客户评价及客户问答

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HEK293 cells)
Specification
HEK293 cells
Treatment
control or CK2 siRNA
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 May 07 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Human Cell (Human primary fibroblasts)
Specification
Human primary fibroblasts
Permeabilization
Yes - 0.2% Triton-X100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 16 2013

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount
20 µg
Specification
Mouse embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 23 2011

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cells)
Total protein in input
200 µg
Specification
HeLa cells
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

提交于 Jan 18 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Treatment
CK2 alpha siRNA
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Dr. Svetlana Khoronenkova

Verified customer

提交于 Jul 23 2010

Thank you for your enquiry and patience. Under the conditions tested, which included WB detection at optimized dilutions using lysate from cells expressing endogenous amounts of the subunits, they were specific as specified. That is, ab10466, ab10468 a...

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Ab10466, ab10468 and ab10470 react with the alpha subunit and ab10474 reacts with the alpha prime subunit. Please contact us again if you have any additional questions.

Thank you for your enquiry. The immunogen information for the products are as follows: Ab10466- The immunogen used lies between aa 1-50. Ab10468- The immunogen used lies between aa 300-350. Ab10470- The immunogen used lies between aa350-391. ...

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Thank you for your enquiry and your interest in our product. This particular antibody has only been for Western blot application and we do not have any information if it works for immunocyto/histochemistry. All tested applications are specified...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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