概述

  • 产品名称Anti-CKAP4抗体
    参阅全部 CKAP4 一抗
  • 描述
    兔多克隆抗体to CKAP4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 553-602 (LRTAVDSLVA YSVKIETNEN NLESAKGLLD DLRNDLDRLF VKVEKIHEKV) of Human CKAP4 (NP_006816).

  • 阳性对照
    • 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-CKAP4 antibody 图像

  • Anti-CKAP4 antibody (ab84705) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 66 kDa

Anti-CKAP4 antibody (ab84705)参考文献

This product has been referenced in:
  • Hoedt E  et al. SILAC-based proteomic profiling of the human MDA-MB-231 metastatic breast cancer cell line in response to the two antitumoral lactoferrin isoforms: the secreted lactoferrin and the intracellular delta-lactoferrin. PLoS One 9:e104563 (2014). WB ; Human . Read more (PubMed: 25116916) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - other (293T)
Specification 293T
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Mar 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"