Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner.
Abundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney.
Belongs to the CKAP2 family.
Present at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C).
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules.
Tumor associated microtubule associated protein antibody
Tumor- and microtubule-associated protein antibody
Anti-CKAP2 antibody 图像
Western blot - CKAP2 antibody (ab85889)
All lanes : Anti-CKAP2 antibody (ab85889) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique
Predicted band size : 77 kDa Observed band size : 77 kDa
Exposure time : 90 seconds
Immunoprecipitation - CKAP2 antibody (ab85889)
Detection of CKAP2 by Western Blot of Immunprecipitate.
ab85889, at 1 µg/ml, staining CKAP2 in HeLa whole cell lysate immunoprecipitated using ab85889 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 90 seconds.