概述

  • 产品名称Anti-CK1 epsilon抗体
    参阅全部 CK1 epsilon 一抗
  • 描述
    兔多克隆抗体to CK1 epsilon
  • 特异性ab70110 detects endogenous levels of total CK1 epsilon protein.
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal sequence of human CK1 epsilon

  • 阳性对照
    • LOVO cell extracts, HeLa cells and human placenta tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab70110 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab70110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.
  • 组织特异性Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
  • 序列相似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Casein kinase 1 epsilon antibody
    • Casein kinase I antibody
    • Casein kinase I epsilon antibody
    • Casein kinase I isoform epsilon antibody
    • CKI epsilon antibody
    • CKI-epsilon antibody
    • CKIe antibody
    • CKIepsilon antibody
    • CSNK 1E antibody
    • CSNK1E antibody
    • DBT antibody
    • Doubletime, Drosophila, homolog of antibody
    • EC 2.7.11.1 antibody
    • epsilon isoform antibody
    • HCK IE antibody
    • KC1E antibody
    • KC1E_HUMAN antibody
    • kinase CK1 epsilon antibody
    • MGC 10398 antibody
    see all

Anti-CK1 epsilon antibody 图像

  • ab70110 at 1/500 dilution staining CK1 epsilon in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab70110 at 1/50 dilution staining CK1 epsilon in human placenta by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide
  • All lanes : Anti-CK1 epsilon antibody (ab70110) at 1/500 dilution

    Lane 1 : LOVO cell extracts
    Lane 2 : LOVO cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 48 kDa
    Observed band size : 48 kDa

Anti-CK1 epsilon antibody (ab70110)参考文献

This product has been referenced in:
  • Scoma HD  et al. The De-Ubiquitinylating Enzyme, USP2, Is Associated with the Circadian Clockwork and Regulates Its Sensitivity to Light. PLoS One 6:e25382 (2011). WB ; Human . Read more (PubMed: 21966515) »

See 1 Publication for this product

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