概述

  • 产品名称Anti-Citrulline抗体
    参阅全部 Citrulline 一抗
  • 描述
    兔多克隆抗体to Citrulline
  • 特异性ab100932 reacts specifically with intrapeptidic citrulline independently of the amino acid sequence. It does not react with free citrulline, ornithine or arginine. Citrulline is an un-natural amino-acid and its expression is very low. Factors limiting the detection are the amount of endogenous citrulline within a protein and the accessibility of the antibody to these sites.
  • 经测试应用适用于: ELISA, ICCmore details
    不适用于: WB
  • 免疫原

    Citrulline coupled to KLH via Glutaraldehyde

  • 阳性对照
    • Hypercitrullinated Histones induced in HL60 cells by DMSO +calcium +calcium ionophore A23187

性能

应用

Our Abpromise guarantee covers the use of ab100932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 相关性The amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation. Citrulline promotes energy and assists with the immune system. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.

    Anti-Citrulline antibody 图像

    • ELISA data showing the high immuno-reactivity of ab100932 against citrulline due to the high amounts of citrulline conjugated to BSA.

    • ELISA data demonstrating the specificity of ab100932 to citrulline naturally present in histones (endogenous citrulline).

    • Immunocytochemical analysis of Human buccal mucosal cells, staining Citrulline with ab100932.

      Cells were fixed with acetone followed by 2% glutaraldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS) for 15 hours at 4°C. An undiluted biotinylated anti-rabbit polyclonal IgG was used as the secondary antibody.

      See Abreview

    Anti-Citrulline antibody (ab100932)参考文献

    This product has been referenced in:
    • Deplus R  et al. Citrullination of DNMT3A by PADI4 regulates its stability and controls DNA methylation. Nucleic Acids Res N/A:N/A (2014). WB . Read more (PubMed: 24957603) »

    See 1 Publication for this product

    Product Wall

    Ab100932 was tested on ELISA on various proteins and peptides containing citrulline and gave positive results. An inhibition with free citrulline, ornithine or arginine was also performed and show no decrease of the signal on citrullinated protein or p...

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    WB can be tried using dilution between 1/500- 1/1000.
    ELISA can tried using 1/500 dilution.
    The information about positive control is given in Hagiwara et. al. 2001.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cell (buccal mucosa)
    Specification buccal mucosa
    Fixative acetone followed by 2% gluteraldehyde
    Permeabilization Yes - 0.1 % triton in blocking buffer
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 29 2013

    I am sorry to hear that this antibody is not providing satisfactory results.

    Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab100932. I would also appreciate if you can confirm some furthe...

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    DISCOUNT CODE: ***

    Expiration date: January 28th, 2013

    I am very pleased to hear you would like to accept our offer and test ab100932 inIHC. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this ...

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    Thank you for your inquiry.

    The lab did not have a positive control testing WB image, however, they were able to pass along the protocol tested:

    The positive control was HL60 cells treated with DMSO or with DMSO and A23187. The hist...

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    Thank you for contacting us. We currently have two antibodies that will detect the citrulline modification on any proteins: ab6464 and ab100932. While ab6464 has been validated in IHC on frozen tissue sections, we have not tested this antibody for use ...

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    Thank you for confirming these details and for your cooperation.

    As requested, I have issued a free of charge replacement with the order number *******.

    To check the status of the order please contact our Customer Service team and ref...

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    Thank you for confirming these details and for your cooperation.

    As agreed, I have issued a free of charge replacement with one unit of ab6464,the order number ******.

    To check the status of the order please contact our Customer Servi...

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    Thank you for your reply.

    The Abpromise guarantee is only valid for products bought direct from Abcam or one of our authorized distributors. The terms and conditions of the guarantee can be found on http://www.abcam.com/abpromise.

    I l...

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