概述

  • 产品名称
    Anti-Citrobacter koseri抗体
  • 描述
    兔多克隆抗体to Citrobacter koseri
  • 特异性
    This antibody targets Citrobacter koseri proteins.
  • 经测试应用
    适用于: ICC/IFmore details
  • 种属反应性
    Reacts with Citrobacter koseri. Not yet tested in other species.
  • 免疫原

    Full length native protein (purified) corresponding to Citrobacter koseri. Citrobacter koseri total proteins

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: None
    Constituents: 50% Glycerol, ddH20
  • Concentration information loading...
  • 纯化说明
    Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab37056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性
    Citrobacter is a genus of gram-negative bacteria in the family of the Enterobacteriaceae. The species C. amalonaticus, C. koseri, and C. freundii use solely citrate as a carbon source. These bacteria can be found almost everywhere in soil, water, wastewater, etc. It can also be found in the human intestine. They are rarely the source of illnesses, except for infections of the urinary tract and infant meningitis.

图片

  • ab37056 staining Citobacter koseri in mouse colon tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Sections were snap frozen without fixation and incubated in 1% PFA in PBS for 10 minutes after cutting sections (5µm). Sections were permeabilized with 0.5% NP40 and blocked with 10% goat serum for 1 hour at 25°C. Sections were incubated with primary antibody (1/500 in PBS+ 5% goat serum) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Singh K  et al. The L-Arginine Transporter Solute Carrier Family 7 Member 2 Mediates the Immunopathogenesis of Attaching and Effacing Bacteria. PLoS Pathog 12:e1005984 (2016). ICC/IF . Read more (PubMed: 27783672) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (mouse colon sections (snap frozen))
Specification
mouse colon sections (snap frozen)
Permeabilization
Yes - 0.5% NP40
Fixative
snap frozen without fixation 1% PFA in PBS for 10min after sections cut
Username

Dr. Jerry Turner

Verified customer

提交于 Feb 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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