概述

  • 产品名称
    Anti-Citrate synthetase抗体
    参阅全部 Citrate synthetase 一抗
  • 描述
    兔多克隆抗体to Citrate synthetase
  • 经测试应用
    适用于: ICC/IF, IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Zebrafish
    预测可用于: Cow, Pig, Xenopus laevis
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 44-316 of Human Citrate synthetase (NP_938083).

  • 阳性对照
    • WB: 293T, A431, HeLa and HepG2 whole cell lysates and zebrafish skeletal muscle tissue and mouse liver mitochondrial homogenates. ICC/IF: HeLa and U2OS cells. IHC-P: Human colon, mouse intestine, mouse muscle and NCI-N87 xenograft tissues. IP: 293T whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab96600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.

靶标

图片

  • All lanes : Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 52 kDa

    10% SDS-PAGE.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Citrate synthetase (green) with ab96600 at a dilution of 1/200. Calls were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Alpha Tubulin (red) was stained with ab184613 at a dilution of 1/1000. Blue: Hoechst 33342 staining.

  • IHC image of ab96600 staining in Human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96600, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunoprecipitation of Citrate synthetase from 293T whole cell lysate using 5 μg of ab96600.

    Western blot analysis was performed using ab96600.

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Citrate synthetase (green) with ab96600 at a dilution of 1/500. Calls were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Alpha Tubulin (red) was stained with ab184613 at a dilution of 1/1000. Blue: Hoechst 33342 staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse intestine tissue labelling Citrate synthetase with ab96600 at a dilution of 1/500.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse muscle tissue labelling Citrate synthetase with ab96600 at a dilution of 1/500.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of NCI-N87 xenograft tissue labelling Citrate synthetase with ab96600 at a dilution of 1/500.

  • All lanes : Anti-Citrate synthetase antibody (ab96600) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish liver homogenate (20ug)
    Lane 3 : Zebrafish skeletal muscle homogenate (20ug)
    Lane 4 : Mouse liver mitochondrial homogenate (20ug)

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

文献

This product has been referenced in:
  • Redmann M  et al. Inhibition of autophagy with bafilomycin and chloroquine decreases mitochondrial quality and bioenergetic function in primary neurons. Redox Biol 11:73-81 (2017). WB . Read more (PubMed: 27889640) »
  • Giroud M  et al. Let-7i-5p represses brite adipocyte function in mice and humans. Sci Rep 6:28613 (2016). Read more (PubMed: 27345691) »

See all 44 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bos taurus Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-15% gradient gel)
Loading amount
20 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Julie Kim

Verified customer

提交于 Apr 17 2017

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (MRC5 normal lung fibroblasts)
Specification
MRC5 normal lung fibroblasts
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

提交于 Feb 13 2015

Application
Western blot
Loading amount
0.5 µg
Gel Running Conditions
Reduced Denaturing (4-15% Tris-glycine)
Sample
Pig Recombinant protein (Citrate Synthase from porcine heart (Sigma))
Specification
Citrate Synthase from porcine heart (Sigma)
Blocking step
Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. Rachel Loh

Verified customer

提交于 Jan 15 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10% 37.5:1)
Sample
Pig Tissue lysate - other (Skeletal muscle mitochondria purified)
Specification
Skeletal muscle mitochondria purified
Blocking step
3% Casein in PBS with 0.1% Tween-20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 27 2014

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (10% 37.5:1)
Sample
Cow Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
3% Casein in PBS with 0.1% Tween-20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 27 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 100mM sodium cytrate
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Hadas Pahima

Verified customer

提交于 Oct 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
30 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ilan Sela

Verified customer

提交于 Aug 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12)
Loading amount
20 µg
Specification
C2C12
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 26 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (L6 myotubes)
Loading amount
20 µg
Specification
L6 myotubes
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Feb 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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