Citrate Assay试剂盒(ab83396)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.002 mM
概述
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产品名称
Citrate Assay试剂盒 -
检测方法
Colorimetric/Fluorometric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Quantitative -
灵敏度
> 0.002 mM -
范围
0.002 mM - 10 mM -
检测时间
0h 40m -
产品概述
Citrate Assay Kit ab83396 provides a simple, sensitive and rapid means of quantifying citrate in biological samples.
In the citrate assay protocol, citrate is converted to pyruvate via oxaloacetate. The pyruvate is quantified by converting a nearly colorless probe to an intensely colored (570 nm) and fluorescent (Ex/Em, 535/587 nm) product.
The citrate assay kit can detect 0.1 to 10 nmoles (~2 µM-10 mM) of citrate.
Citrate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K655 Citrate Colorimetric/Fluorometric Assay Kit. K655-100 is the same size as the 100 test size of ab83396.
Citric acid (HOOC-CH2-C(-OH)(-COOH)-CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Citrate Assay Buffer 1 x 25ml Citrate Developer 1 vial Citrate Enzyme Mix 1 vial Citrate Standard 1 vial OxiRed Probe 1 x 0.2ml -
研究领域
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相关性
Citric acid (HOOC-CH2-C(-OH)(-COOH)-CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis (acetyl-CoA carboxylase) and glycolysis (phospho-fructokinase). Citrate is widely used industrially in foods, beverages and pharmaceuticals. Citrate metabolism and disposition can vary widely due to sex, age and a variety of other factors. -
别名
- Citric acid
相关产品
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图片
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab83396 被引用在 12 文献中.
- Song X et al. PDK4 dictates metabolic resistance to ferroptosis by suppressing pyruvate oxidation and fatty acid synthesis. Cell Rep 34:108767 (2021). PubMed: 33626342
- Nagandla H et al. Isoform-specific Activities of Androgen Receptor and its Splice Variants in Prostate Cancer Cells. Endocrinology 162:N/A (2021). PubMed: 33300995
- Son SM et al. Leucine regulates autophagy via acetylation of the mTORC1 component raptor. Nat Commun 11:3148 (2020). PubMed: 32561715
- Ding H et al. NONO promotes hepatocellular carcinoma progression by enhancing fatty acids biosynthesis through interacting with ACLY mRNA. Cancer Cell Int 20:425 (2020). PubMed: 32884448
- Lou PH et al. Lipid Emulsion Containing High Amounts of n3 Fatty Acids (Omegaven) as Opposed to n6 Fatty Acids (Intralipid) Preserves Insulin Signaling and Glucose Uptake in Perfused Rat Hearts. Anesth Analg 130:37-48 (2020). PubMed: 31274599