重组Anti-CITED2抗体[EPR3416(2)] (ab108345)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3416(2)] to CITED2
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-CITED2抗体[EPR3416(2)]
参阅全部 CITED2 一抗 -
描述
兔单克隆抗体[EPR3416(2)] to CITED2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IP -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Saos-2, U937, NIH/3T3 and HeLa whole cell lysate (ab150035).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3416(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108345于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
靶标
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功能
Interferes with the binding of transcription factors HIF-1a and STAT2 to p300/CBP. -
序列相似性
Belongs to the CITED family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10370 Human
- Entrez Gene: 17684 Mouse
- Omim: 602937 Human
- SwissProt: Q99967 Human
- SwissProt: O35740 Mouse
- Unigene: 82071 Human
- Unigene: 272321 Mouse
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别名
- ASD8 antibody
- Cbp/p300 interacting transactivator 2 antibody
- Cbp/p300 interacting transactivator with Glu/Asp rich carboxy terminal domain 2 antibody
see all
图片
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Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 1/2000 dilution (purified) + NIH/3T3 whole cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 20 µg (purified) + Saos-2 whole cell lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 1/1000 dilution (purified) + U937 whole cell lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 1/1000 dilution (unpurified)
Lane 1 : Saos-2 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 28 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab108345 被引用在 8 文献中.
- Huang T et al. The pro-death role of Cited2 in stroke is regulated by E2F1/4 transcription factors. J Biol Chem 294:8617-8629 (2019). PubMed: 30967472
- Yuan Y et al. MAT2B promotes proliferation and inhibits apoptosis in osteosarcoma by targeting epidermal growth factor receptor and proliferating cell nuclear antigen. Int J Oncol 54:2019-2029 (2019). PubMed: 30942439
- Collett GP et al. Endoplasmic reticulum stress stimulates the release of extracellular vesicles carrying danger-associated molecular pattern (DAMP) molecules. Oncotarget 9:6707-6717 (2018). PubMed: 29467921
- AlAbdi L et al. The transcription factor Vezf1 represses the expression of the antiangiogenic factor Cited2 in endothelial cells. J Biol Chem 293:11109-11118 (2018). PubMed: 29794136
- Imakawa K et al. CITED2 modulation of trophoblast cell differentiation: insights from global transcriptome analysis. Reproduction 151:509-16 (2016). PubMed: 26917451
- Sakai M et al. The GCN5-CITED2-PKA signalling module controls hepatic glucose metabolism through a cAMP-induced substrate switch. Nat Commun 7:13147 (2016). Mouse . PubMed: 27874008
- Fame RM et al. Cited2 Regulates Neocortical Layer II/III Generation and Somatosensory Callosal Projection Neuron Development and Connectivity. J Neurosci 36:6403-19 (2016). PubMed: 27307230
- Saito Y et al. High-Throughput siRNA Screening to Reveal GATA-2 Upstream Transcriptional Mechanisms in Hematopoietic Cells. PLoS One 10:e0137079 (2015). PubMed: 26325290