Mouse, Rat, Cow, Pig, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CITED2.
- This antibody gave a positive signal in the following whole cell lysates: Caco2; SW13; Saos2; HeLa; Jurkat.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Interferes with the binding of transcription factors HIF-1a and STAT2 to p300/CBP.
Belongs to the CITED family.
Information by UniProt
- ASD8 antibody
- Cbp/p300 interacting transactivator 2 antibody
- Cbp/p300 interacting transactivator with Glu/Asp rich carboxy terminal domain 2 antibody
Western blot - CITED2 antibody (ab99335)
All lanes :
Anti-CITED2 antibody (ab99335) at 1 µg/mlLane 1 :
Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLane 2 :
SW13 (Human adrenal adenocarcinoma) Whole Cell LysateLane 3 :
Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell LysateLane 4 :
HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 5 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.Predicted band size :
28 kDaObserved band size :
28 kDaAdditional bands at :
120 kDa,26 kDa (possible isoform),55 kDa. We are unsure as to the identity of these extra bands.Exposure time :
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"