概述

  • 产品名称Anti-CITED2抗体
    参阅全部 CITED2 一抗
  • 描述
    兔多克隆抗体to CITED2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CITED2.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Caco2; SW13; Saos2; HeLa; Jurkat.

性能

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应用

Our Abpromise guarantee covers the use of ab99335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

靶标

Anti-CITED2 antibody 图像

  • All lanes : Anti-CITED2 antibody (ab99335) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : SW13 (Human adrenal adenocarcinoma) Whole Cell Lysate
    Lane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 120 kDa,26 kDa (possible isoform),55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-CITED2 antibody (ab99335)参考文献

ab99335 has not yet been referenced specifically in any publications.

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