概述

  • 产品名称Anti-CIT抗体
    参阅全部 CIT 一抗
  • 描述
    兔多克隆抗体to CIT
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Chicken, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide, from a region within amino acids 1925-1975 of Human CIT (NP_009105.1)

  • 阳性对照
    • HeLa whole cell lysate 293T whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab86782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 10µg/mg of lysate.
    WB: 1/2000 - 1/10000 (or 0.1 - 1µg/ml). Predicted molecular weight: 231 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性CIT is a putative RHO/RAC effector that binds to the GTP bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. This protein exhibits dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system.
    • 细胞定位Cytoplasmic
    • 数据库链接
    • 别名
      • Citron (rho interacting serine/threonine kinase 21) antibody
      • Citron antibody
      • Citron Rho interacting kinase antibody
      • CRIK antibody
      • EC 2.7.11.1 antibody
      • KIAA0949 antibody
      • Rho interacting serine/threonine kinase 21 antibody
      • Rho interacting serine/threonine protein kinase 21 antibody
      • serine/threonine-protein kinase 21 antibody
      • STK2 antibody
      • STK21 antibody
      see all

    Anti-CIT antibody 图像

    • All lanes : Anti-CIT antibody (ab86782) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 231 kDa


      Exposure time : 30 seconds
    • 1 mg HeLa whole cell lysate was immunoprecipitated with 10 µg ab86782 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was loaded onto a Western blot and labelled with ab86782 at 1 µg/ml. Bands were detected by chemiluminescence with an exposure time of 10 seconds.

    Anti-CIT antibody (ab86782)参考文献

    ab86782 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab86782.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"