Anti-CIT抗体(ab86782)
Key features and details
- Rabbit polyclonal to CIT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CIT抗体
参阅全部 CIT 一抗 -
描述
兔多克隆抗体to CIT -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Horse, Chicken, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
免疫原
Synthetic peptide corresponding to Human CIT aa 1900-2000.
Database link: O14578 -
阳性对照
- HeLa whole cell lysate (ab150035) 293T whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab86782于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 231 kDa.
Or 0.1 - 1µg/ml. |
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IP |
Use at 10 µg/mg of lysate.
|
说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 231 kDa. Or 0.1 - 1µg/ml. |
IP
Use at 10 µg/mg of lysate. |
靶标
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功能
Plays a role in cytokinesis. Required for KIF14 localization to the central spindle and midbody. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. Displays serine/threonine protein kinase activity. Plays an important role in the regulation of cytokinesis and the development of the central nervous system. Phosphorylates MYL9/MLC2. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 CNH domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 11113 Human
- Omim: 605629 Human
- SwissProt: O14578 Human
- Unigene: 119594 Human
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别名
- CIT antibody
- Citron (rho interacting serine/threonine kinase 21) antibody
- Citron antibody
see all
图片
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All lanes : Anti-CIT antibody (ab86782) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 231 kDa
Exposure time: 30 seconds -
1 mg HeLa whole cell lysate was immunoprecipitated with 10 µg ab86782 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was loaded onto a Western blot and labelled with ab86782 at 1 µg/ml. Bands were detected by chemiluminescence with an exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab86782 被引用在 3 文献中.
- Lavado-García J et al. Metabolic engineering of HEK293 cells to improve transient transfection and cell budding of HIV-1 virus-like particles. Biotechnol Bioeng 118:1649-1663 (2021). PubMed: 33463716
- Liu Z et al. Novel circRNA_0071196/miRNA-19b-3p/CIT axis is associated with proliferation and migration of bladder cancer. Int J Oncol 57:767-779 (2020). PubMed: 32705161
- Sgrò F et al. Tissue-specific control of midbody microtubule stability by Citron kinase through modulation of TUBB3 phosphorylation. Cell Death Differ 23:801-13 (2016). PubMed: 26586574