Anti-Cisplatin modified DNA抗体[CP9/19] (ab103261)

概述

  • 产品名称
    Anti-Cisplatin modified DNA抗体[CP9/19]
  • 描述
    大鼠单克隆抗体[CP9/19] to Cisplatin modified DNA
  • 宿主
    Rat
  • 经测试应用
    适用于: ELISA, Flow Cyt, Dot blotmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Cisplatin Modified Native DNA.

  • 阳性对照
    • RNA/DNA.
  • 常规说明


    This antibody enables the quantification of cisplatin-induced adducts on DNA. It has also been recently used for isolation of DNA fragments carrying adducts to enhance the sensitivity of subsequent PCR-based analyses and is central to ongoing studies of variation in the nature of cisplatin adducts formed in different cell lines.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Primary antibody说明
    This antibody enables the quantification of cisplatin-induced adducts on DNA. It has also been recently used for isolation of DNA fragments carrying adducts to enhance the sensitivity of subsequent PCR-based analyses and is central to ongoing studies of variation in the nature of cisplatin adducts formed in different cell lines.
  • 克隆
    单克隆
  • 克隆编号
    CP9/19
  • 骨髓瘤
    Y3/Ag1.2.3
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab103261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 23370278

ab18450 - Rat IgG2a kappa monoclonal, is suitable for use as an isotype control with this antibody.

Dot blot Use at an assay dependent concentration. PubMed: 25356874

图片

  • ab103261 staining Cisplatin modified DNA in human ovarian cancer cell line A2780 by Flow Cytometry. Cells in culture were detached by accutase treatment, fixed with 70% ehtanol for 30 min at 4°C and permeabilized with 100ug/ml digitonin in PBS. The sample was incubated with the primary antibody (1/200 in PBS + 1% FBS) for 18 hours at 4°C. A FITC-conjugated rabbit anti-rat IgG H&L polyclonal (1/200) was used as the secondary antibody. Gating Strategy: FITC positive cells.

    The sh2 represents the gene knock down cell line which showed positive staining with anti-cisplatin adduct antibody upon cisplatin treatment, whereas the control cells (cis-R, resistant to cisplatin) did not show any staining with anti-cis adduct antibody upon cisplatin treatment.

    See Abreview

文献

This product has been referenced in:
  • Jarrett SG  et al. The melanocortin signaling cAMP axis accelerates repair and reduces mutagenesis of platinum-induced DNA damage. Sci Rep 7:11708 (2017). Read more (PubMed: 28916831) »
  • Han C  et al. Differential DNA lesion formation and repair in heterochromatin and euchromatin. Carcinogenesis 37:129-38 (2016). Read more (PubMed: 26717995) »

See all 9 Publications for this product

客户评价及客户问答

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Application
Flow Cytometry
Sample
Human Cell (human ovarian cancer cell line A2780)
Permeabilization
Yes - 100ug/ml digitonin in PBS
Gating Strategy
FITC positive cells
Specification
human ovarian cancer cell line A2780
Preparation
Cell harvesting/tissue preparation method: Cells in culture detached by accutase treatment
Sample buffer: PBS
Fixation
70% ehtanol for 30 min at 4 degrees
Username

Abcam user community

Verified customer

提交于 Apr 16 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (ovarian cancer A2780 cell line)
Specification
ovarian cancer A2780 cell line
Permeabilization
Yes - 0.1% digitonin in PBS
Fixative
70% ethanol
Username

Abcam user community

Verified customer

提交于 Aug 25 2014

Application
ELISA
Sample
Human Cell (melanoma)
Specification
melanoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.74µg/mL · Temperature: 20°C
Type
Direct
Username

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Verified customer

提交于 May 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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