• 产品名称Anti-CISH抗体
    参阅全部 CISH 一抗
  • 描述
    小鼠多克隆抗体to CISH
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human CISH protein (NP_659508.1)

  • 阳性对照
    • Human spleen lysate. CISH transfected 293T cell lysate. HeLa cells.



Our Abpromise guarantee covers the use of ab88383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
ICC/IF Use a concentration of 10 µg/ml.


  • 功能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • 组织特异性Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • 翻译后修饰Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
  • Information by UniProt
  • 数据库链接
  • 别名
    • BACTS2 antibody
    • cis 1 antibody
    • CIS antibody
    • CIS-1 antibody
    • CIS1 antibody
    • Cish antibody
    • CISH_HUMAN antibody
    • Cytokine inducible inhibitor of signaling type 1B antibody
    • Cytokine inducible SH2 containing protein antibody
    • Cytokine-inducible SH2-containing protein antibody
    • G18 antibody
    • Protein G18 antibody
    • SOCS antibody
    • Suppressor of cytokine signaling antibody
    see all

Anti-CISH antibody 图像

  • Anti-CISH antibody (ab88383) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size : 29 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CISH antibody (ab88383) at 1 µg/ml

    Lane 1 : CISH transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size : 29 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of CISH in HeLa cells using ab88383 at 10µg/ml.

Anti-CISH antibody (ab88383)参考文献

ab88383 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

I am sorry to hear that the antibody is not working as previously.

As we discussed over the phone, I have arranged for a free of charge replacement with a new lot number (GR89220-1) which you will receiv...

Read More

Thank you for contacting us.

I checked, but we did not unpublish any CISH antibody in the last couple years.
Also, I did not see any changes made to the MW. Our internal notes do not indicate that.

The predicted MWfor human CI...

Read More