参阅全部 CISD1 一抗
小鼠单克隆抗体[OTI2B3] to CISD1
WB, Flow Cyt, ICC/IFmore details
Recombinant full length Human CISD1 produced in HEK293T cells (NP_060934).
- HEK293T cell lysate transfected with pCMV6-ENTRY CISD1 cDNA;
COS7 cells transiently transfected by pCMV6-ENTRY CISD1; Jurkat cells.
Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.
The clone number has been updated from 2B3 to OTI2B3, both clone numbers name the same clone.
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- 存储溶液pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA
Concentration information loading...
- 纯度Protein G purified
- 纯化说明ab118027 was purified from Mouse ascites fluids by affinity chromatography.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500. Predicted molecular weight: 12 kDa.
||1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
- 功能Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions.
- 组织特异性Expression is reduced in cells derived from cystic fibrosis patients.
- 序列相似性Belongs to the CISD protein family.
- 细胞定位Mitochondrion outer membrane.
Information by UniProt
- AU043990 antibody
- AW743335 antibody
- C10orf70 antibody
Anti-CISD1 antibody [OTI2B3] 图像
Western blot - Anti-CISD1 antibody [OTI2B3] (ab118027)
All lanes : Anti-CISD1 antibody [OTI2B3] (ab118027) at 1/500 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CISD1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size : 12 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203308)
Immunocytochemistry/ Immunofluorescence - Anti-CISD1 antibody [OTI2B3] (ab118027)
ab118027, at 1/100 dilution, staining CISD1 in COS7 cells transiently transfected by pCMV6-ENTRY CISD1 by Immunofluorescence.
Flow Cytometry - Anti-CISD1 antibody [OTI2B3] (ab118027)
Flow cytometric Analysis of CISD1 staining in Jurkat cells, using ab118027 at 1/100 dilution(Red), compared to a nonspecific negative control antibody(Blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"