概述

  • 产品名称Anti-CISD1抗体[2B3]
    参阅全部 CISD1 一抗
  • 描述
    小鼠单克隆抗体[2B3] to CISD1
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human CISD1 produced in HEK293T cells (NP_060934).

  • 阳性对照
    • HEK293T cell lysate transfected with pCMV6-ENTRY CISD1 cDNA; COS7 cells transiently transfected by pCMV6-ENTRY CISD1; Jurkat cells.
  • 常规说明Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

性能

应用

Our Abpromise guarantee covers the use of ab118027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Predicted molecular weight: 12 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

靶标

  • 功能Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions.
  • 组织特异性Expression is reduced in cells derived from cystic fibrosis patients.
  • 序列相似性Belongs to the CISD protein family.
  • 细胞定位Mitochondrion outer membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AU043990 antibody
    • AW743335 antibody
    • C10orf70 antibody
    • CDGSH iron sulfur domain 1 antibody
    • CDGSH iron-sulfur domain-containing protein 1 antibody
    • CISD 1 antibody
    • CISD1 antibody
    • CISD1_HUMAN antibody
    • D10Ertd214e antibody
    • MDS029 antibody
    • MGC14684 antibody
    • MitoNEET antibody
    • RGD1309529 antibody
    • ZCD1 antibody
    • Zinc finger CDGSH type domain 1 antibody
    see all

Anti-CISD1 antibody [2B3] 图像

  • All lanes : Anti-CISD1 antibody [2B3] (ab118027) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CISD1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 12 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203308)
  • ab118027, at 1/100 dilution, staining CISD1 in COS7 cells transiently transfected by pCMV6-ENTRY CISD1 by Immunofluorescence.
  • Flow cytometric Analysis of CISD1 staining in Jurkat cells, using ab118027 at 1/100 dilution(Red), compared to a nonspecific negative control antibody(Blue).

Anti-CISD1 antibody [2B3] (ab118027)参考文献

This product has been referenced in:
  • Salem AF  et al. Mitochondrial biogenesis in epithelial cancer cells promotes breast cancer tumor growth and confers autophagy resistance. Cell Cycle 11:4174-80 (2012). Read more (PubMed: 23070475) »

See 1 Publication for this product

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