Anti-CIRBP抗体(ab106230)

概述

  • 产品名称Anti-CIRBP抗体
    参阅全部 CIRBP 一抗
  • 描述
    山羊多克隆抗体to CIRBP
  • 经测试应用适用于: IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Chicken, Cow, Dog, Human, Cynomolgus Monkey, Rainbow Trout, Chinese Hamster
  • 免疫原

    Synthetic peptide:

    C-RDSYDSYATHNE

    , corresponding to C terminal amino acids 161-172 of Human CIRBP (NP_001271.1)

  • 阳性对照
    • Mouse or Rat testis lysate

性能

应用

Our Abpromise guarantee covers the use of ab106230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/20.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.
  • 组织特异性Ubiquitous.
  • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
  • 结构域Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression.
  • 翻译后修饰Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs.
    Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.
  • 细胞定位Nucleus > nucleoplasm. Cytoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A18 hnRNP antibody
    • A18HNRNP antibody
    • cirbp antibody
    • CIRBP_HUMAN antibody
    • CIRP antibody
    • Cold inducible RNA binding protein antibody
    • Cold-inducible RNA-binding protein antibody
    • Glycine rich RNA binding protein antibody
    • Glycine-rich RNA-binding protein CIRP antibody
    • testicular tissue protein Li 39 antibody
    see all

Anti-CIRBP antibody 图像

  • Anti-CIRBP antibody (ab106230) at 0.01 µg/ml + mouse testis lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 19 kDa
  • ab106230 (3.8µg/ml) staining of paraffin embedded Human Testis shows nuclear staining preferentially in type A spermatogonia. Samples steamed antigen retrieval with citrate buffer pH 6 followed by AP-staining.

Anti-CIRBP antibody (ab106230)参考文献

ab106230 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Tissue lysate - whole (Cardiomyocytes)
Total protein in input 30 µg
Specification Cardiomyocytes
Immuno-precipitation step Other - Protein G coated Dynabeads
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提交于 Sep 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"