The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).
Use at 2-5 µg/mg of lysate.
Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.
Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea.
Belongs to the FNBP1 family. Contains 1 FCH domain. Contains 1 REM (Hr1) repeat. Contains 1 SH3 domain.
Tyrosine phosphorylated. Also phosphorylated by PKA.
Cytoplasm > perinuclear region and Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Lysosome. Golgi apparatus. Cell membrane. Cell projection > phagocytic cup. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity). Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9.
All lanes : Anti-Cip4 antibody (ab72220) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Detection of Human Cip4 by Immunoprecipitation. 1mg HeLa whole cell lysate loaded in lanes 1-3 with an antibody recognising an upstream epitope in lane 1, ab72220 at 3 µg in lane 2 and control IgG in lane 3. Image shows immunoprecipitated Cip4 detected using post IP WB, loading 20% of IP and using ab72220 at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.