概述

  • 产品名称Anti-Cip4抗体
    参阅全部 Cip4 一抗
  • 描述
    兔多克隆抗体to Cip4
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    A region between residues 551 and 601 of human Cip4

  • 阳性对照
    • HeLa cytoplasmic or membrane lysate 293T cytoplasmic or membrane lysate Mouse NIH3T3 cytoplasmic or membrane lysate

性能

应用

Our Abpromise guarantee covers the use of ab72220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.
  • 组织特异性Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea.
  • 序列相似性Belongs to the FNBP1 family.
    Contains 1 FCH domain.
    Contains 1 REM (Hr1) repeat.
    Contains 1 SH3 domain.
  • 翻译后修饰Tyrosine phosphorylated. Also phosphorylated by PKA.
  • 细胞定位Cytoplasm > perinuclear region and Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Lysosome. Golgi apparatus. Cell membrane. Cell projection > phagocytic cup. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity). Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cdc42 interacting protein 4 antibody
    • Cdc42 interaction protein 4 long isoform antibody
    • Cdc42-interacting protein 4 antibody
    • CG11341 antibody
    • CG15015 PA antibody
    • Cip 4 antibody
    • CIP4 antibody
    • CIP4_HUMAN antibody
    • DCIP4 antibody
    • hSTP antibody
    • Protein Felic antibody
    • Salt tolerant protein antibody
    • Salt tolerator antibody
    • STOT antibody
    • STP antibody
    • Thyroid hormone receptor interactor 10 antibody
    • Thyroid receptor interacting protein 10 antibody
    • Thyroid receptor-interacting protein 10 antibody
    • TR-interacting protein 10 antibody
    • TRIP 10 antibody
    • TRIP-10 antibody
    • trip10 antibody
    • Truncated Cdc42 interaction protein 4 antibody
    see all

Anti-Cip4 antibody 图像

  • All lanes : Anti-Cip4 antibody (ab72220) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds
  • Detection of Human Cip4 by Immunoprecipitation. 1mg HeLa whole cell lysate loaded in lanes 1-3 with an antibody recognising an upstream epitope in lane 1, ab72220 at 3 µg in lane 2 and control IgG in lane 3. Image shows immunoprecipitated Cip4 detected using post IP WB, loading 20% of IP and using ab72220 at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-Cip4 antibody (ab72220)参考文献

ab72220 has not yet been referenced specifically in any publications.

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