Anti-CIP29抗体(ab67569)

概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to CIP29
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length CIP29 protein (Human)

  • 阳性对照
    • CIP29 transfected 293T cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab67569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 24 kDa).

靶标

  • 功能
    Binds both single-stranded and double-stranded DNA with higher affinity for the single-stranded form. Specifically binds to scaffold/matrix attachment region DNA. Also binds single-stranded RNA. Enhances RNA unwinding activity of DDX39A. May participate in important transcriptional or translational control of cell growth, metabolism and carcinogenesis.
  • 组织特异性
    Low expression in spleen, liver, pancreas, testis, thymus, heart, and kidney. Increased levels are seen in hepatocellular carcinoma and pancreatic adenocarcinoma.
  • 序列相似性
    Contains 1 SAP domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytokine induced protein of 29 kDa antibody
    • Cytokine-induced protein of 29 kDa antibody
    • HCC 1 antibody
    • HCC1 antibody
    • HSPC316 antibody
    • Nuclear protein Hcc 1 antibody
    • Nuclear protein Hcc-1 antibody
    • Proliferation associated cytokine inducible protein CIP29 antibody
    • Proliferation-associated cytokine-inducible protein CIP29 antibody
    • SAP domain-containing ribonucleoprotein antibody
    • SARNP antibody
    • SARNP_HUMAN antibody
    • THO1 antibody
    see all

图片

  • All lanes : Anti-CIP29 antibody (ab67569) at 1/500 dilution

    Lane 1 : CIP29 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg/ml per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 24 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)

文献

ab67569 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS/0.1% Triton
Username

Dr. Nicole Verkaik

Verified customer

提交于 Feb 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
20 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Nicole Verkaik

Verified customer

提交于 Feb 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册