概述

  • 产品名称
    Anti-Cingulin抗体
    参阅全部 Cingulin 一抗
  • 描述
    兔多克隆抗体to Cingulin
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 200-250 of Human Cingulin (NP_065821.1).

  • 阳性对照
    • 293T, Jurkat, and HeLa whole cell lysates

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab117796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能
    Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
  • 组织特异性
    Localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart.
  • 序列相似性
    Belongs to the cingulin family.
  • 结构域
    Deletion of the ZO-1 interaction motif (ZIM) decreases but does not abolish colocalization with ZO-1.
  • 细胞定位
    Cell junction > tight junction. Localizes to the apical junction complex composed of tight and adherens junctions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6330408J11Rik antibody
    • AI647528 antibody
    • AI987749 antibody
    • cgn antibody
    • CING_HUMAN antibody
    • Cingulin antibody
    • DKFZp779N1112 antibody
    • FLJ39281 antibody
    • KIAA1319 antibody
    • MGC118157 antibody
    see all

图片

  • All lanes : Anti-Cingulin antibody (ab117796) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg/ml
    Lane 2 : 293T whole cell lysate at 15 µg/ml
    Lane 3 : Jurkat whole cell lysate at 50 µg/ml
    Lane 4 : HeLa whole cell lysate at 50 µg/ml

    Developed using the ECL technique

    Predicted band size : 136 kDa


    Exposure time : 10 seconds
  • ab117796 at 1 µg/ml staining Cingulin in 293T cell lysate immunoprecipitated using ab117796 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds

文献

ab117796 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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