Anti-Cingulin抗体(ab117796)
Key features and details
- Rabbit polyclonal to Cingulin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cingulin抗体
参阅全部 Cingulin 一抗 -
描述
兔多克隆抗体to Cingulin -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla -
免疫原
Synthetic peptide, corresponding to a region between amino acids 200-250 of Human Cingulin (NP_065821.1).
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阳性对照
- 293T, Jurkat, and HeLa whole cell lysate (ab150035)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117796于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 136 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. -
组织特异性
Localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart. -
序列相似性
Belongs to the cingulin family. -
结构域
Deletion of the ZO-1 interaction motif (ZIM) decreases but does not abolish colocalization with ZO-1. -
细胞定位
Cell junction > tight junction. Localizes to the apical junction complex composed of tight and adherens junctions. - Information by UniProt
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数据库链接
- Entrez Gene: 57530 Human
- Omim: 609473 Human
- SwissProt: Q9P2M7 Human
- Unigene: 591464 Human
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别名
- 6330408J11Rik antibody
- AI647528 antibody
- AI987749 antibody
see all
图片
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All lanes : Anti-Cingulin antibody (ab117796) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg/ml
Lane 2 : 293T whole cell lysate at 15 µg/ml
Lane 3 : Jurkat whole cell lysate at 50 µg/ml
Lane 4 : HeLa whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 136 kDa
Exposure time: 10 seconds -
ab117796 at 1 µg/ml staining Cingulin in 293T cell lysate immunoprecipitated using ab117796 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab117796 被引用在 1 文献中.
- Soroosh A et al. miR-24 Is Elevated in Ulcerative Colitis Patients and Regulates Intestinal Epithelial Barrier Function. Am J Pathol 189:1763-1774 (2019). PubMed: 31220450