Anti-Cingulin抗体(ab117778)

概述

  • 产品名称Anti-Cingulin抗体
    参阅全部 Cingulin 一抗
  • 描述
    兔多克隆抗体to Cingulin
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 350-400 of Human Cingulin (NP_065821.1).

  • 阳性对照
    • Jurkat, HeLa and 293T whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab117778 was affinity purified using an epitope specific to Cingulin immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab117778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
  • 组织特异性Localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart.
  • 序列相似性Belongs to the cingulin family.
  • 结构域Deletion of the ZO-1 interaction motif (ZIM) decreases but does not abolish colocalization with ZO-1.
  • 细胞定位Cell junction > tight junction. Localizes to the apical junction complex composed of tight and adherens junctions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6330408J11Rik antibody
    • AI647528 antibody
    • AI987749 antibody
    • cgn antibody
    • CING_HUMAN antibody
    • Cingulin antibody
    • DKFZp779N1112 antibody
    • FLJ39281 antibody
    • KIAA1319 antibody
    • MGC118157 antibody
    see all

Anti-Cingulin antibody 图像

  • All lanes : Anti-Cingulin antibody (ab117778) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysates at 50 µg
    Lane 2 : 293T whole cell lysates at 15 µg
    Lane 3 : Jurkat whole cell lysates at 50 µg
    Lane 4 : HeLa whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 136 kDa


    Exposure time : 3 minutes
  • Detection of Cingulin by Western Blot of Immunprecipitate.
    ab117778 at 1µg/ml staining Cingulin in 293T whole cell lysate immunoprecipitated using ab117778 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-Cingulin antibody (ab117778)参考文献

ab117778 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"