Anti-Ciliated Cell Marker抗体[LhS28] (ab49852)

概述

  • 产品名称Anti-Ciliated Cell Marker抗体[LhS28]
  • 描述
    小鼠单克隆抗体[LhS28] to Ciliated Cell Marker
  • 经测试应用适用于: IHC-Fr, WB, ICC, Electron Microscopymore details
  • 种属反应性
    与反应: Hamster, Cow, Human
  • 免疫原

    Tissue/ cell preparation - BHK a21 cytoskeletal preparation.

  • 阳性对照
    • BHK a21 cells
  • 常规说明


    This antibody may be used as a tool in the detection of ciliated cells in normal and pathalogical human specimens and for investigating mechanisms of ciliogenesis.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • Primary antibody说明This antibody may be used as a tool in the detection of ciliated cells in normal and pathalogical human specimens and for investigating mechanisms of ciliogenesis.
  • 克隆单克隆
  • 克隆编号LhS28
  • 骨髓瘤Sp2/0
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 44-45 kDa.
ICC Use a concentration of 1 - 2 µg/ml.
Electron Microscopy Use at an assay dependent concentration.

靶标

  • 相关性This protein is expressed in the sub apical zone of cilliated epithelial cells of the Fallopian tube and upper respiratory tract, but not in ciliated ependyma, non ciliated epithelia or testis containing developing spermatozoa. This protein is associated with the basal body structure of the cilium and specifically with the 9+0 microtubule arrays.

Anti-Ciliated Cell Marker antibody [LhS28] 图像

  • ab49852 staining Ciliated Cell Marker in Cow oviduct tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized in ice for 10 minutes and blocked with 5% serum for 30 minutes at 21°C. Samples were incubated with primary antibody (1/400 in 1% BSA in PBST (PBS 0.2% Tween)) for 1 hour at 21°C. A HRP-conjugated goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

Anti-Ciliated Cell Marker antibody [LhS28] (ab49852)参考文献

ab49852 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Sample Cow Tissue sections (Oviduct)
Specification Oviduct
Permeabilization No
Fixative Acetone
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提交于 Jan 06 2014

Thank you very much for your interest in our antibodies.

Our antibody ab49852 is suitable for use as a ciliated cell marker, but has not been validated for use in Flow Cytometry. This antibody also contains Sodium Azide, which would not be s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"