概述

  • 产品名称
  • 描述
    兔多克隆抗体to CIITA
  • 特异性
    This antibody recognizes CIITA.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Dog
  • 免疫原

    A region within synthetic peptide: MALWESLQQQ GEAQLLQAAE EKFTIEPFKA KSPKDVEDLD RLVQTQRLRN, corresponding to amino acids 918-967 of Mouse CIITA

  • 阳性对照
    • NIH3T3 cell lysate
  • 常规说明


    Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 125 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro).
  • 疾病相关
    Bare lymphocyte syndrome 2
  • 序列相似性
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • 结构域
    The acetyltransferase domain is necessary for activation of both class I and class II transcription.
    The GTP-binding motif doesn't confer GTPase activity but promotes nuclear localization.
  • 翻译后修饰
    Autophosphorylated, affecting interaction with TAF7.
  • 细胞定位
    Nucleus. Nucleus, PML body. Recruited to PML body by PML.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C2TA antibody
    • C2TA_HUMAN antibody
    • CIITA antibody
    • CIITA IV antibody
    • Class II major histocompatibility complex transactivator antibody
    • Class II transactivator antibody
    • MHC class II transactivator antibody
    • MHC class II transactivator type III antibody
    • MHC2TA antibody
    • NLR family acid domain containing antibody
    • NLRA antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and acid domain containing antibody
    see all

图片

  • Anti-CIITA antibody (ab49132) at 0.125 µg/ml + NIH3T3 lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 125 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)

实验方案

文献

This product has been referenced in:
  • Kitamura N  et al. Role of PU.1 in MHC class II expression through transcriptional regulation of class II transactivator pI in dendritic cells. J Allergy Clin Immunol 129:814-824.e6 (2012). Read more (PubMed: 22112519) »

See 1 Publication for this product

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