Anti-CIITA抗体(ab117598)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to CIITA
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide from N terminal region of Human CIITA conjugated to KLH

  • 阳性对照
    • Human tonsil tissue

性能

应用

Our Abpromise guarantee covers the use of ab117598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 123 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro).
  • 疾病相关
    Bare lymphocyte syndrome 2
  • 序列相似性
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • 结构域
    The acetyltransferase domain is necessary for activation of both class I and class II transcription.
    The GTP-binding motif doesn't confer GTPase activity but promotes nuclear localization.
  • 翻译后修饰
    Autophosphorylated, affecting interaction with TAF7.
  • 细胞定位
    Nucleus. Nucleus, PML body. Recruited to PML body by PML.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C2TA antibody
    • C2TA_HUMAN antibody
    • CIITA antibody
    • CIITA IV antibody
    • Class II major histocompatibility complex transactivator antibody
    • Class II transactivator antibody
    • MHC class II transactivator antibody
    • MHC class II transactivator type III antibody
    • MHC2TA antibody
    • NLR family acid domain containing antibody
    • NLRA antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and acid domain containing antibody
    see all

图片

  • ab117598, at 5µg/ml, staining CIITA in Human tonsil tissue. The sample was FormalinFixed and Paraffin-Embedded (FFPE).

文献

ab117598 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval (S1699), Dako Target Retrieval pH9 (S2367), or ThermoFisher EDTA Buffer (AP-9004-125) - all tested using steamer for 40 minutes
Permeabilization
No
Specification
Spleen
Blocking step
(agent) for 15 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formalin
Username

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提交于 Dec 01 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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