Anti-CID抗体(ab10887)
Key features and details
- Rabbit polyclonal to CID
- Suitable for: WB, ICC/IF
- Reacts with: Drosophila melanogaster
- Isotype: IgG
概述
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产品名称
Anti-CID抗体 -
描述
兔多克隆抗体to CID -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Drosophila melanogaster -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Drosophila Embryo Lysate;
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10887于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).
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ICC/IF |
1/100.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa). |
ICC/IF
1/100. |
靶标
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功能
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. -
序列相似性
Belongs to the histone H3 family. -
细胞定位
Nucleus. Chromosome, centromere, kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Co-expressed with yemalpha. - Information by UniProt
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数据库链接
- Entrez Gene: 36495 Drosophila melanogaster
- SwissProt: Q9V6Q2 Drosophila melanogaster
- Unigene: 5691 Drosophila melanogaster
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别名
- CENP A antibody
- CENP-A homolog antibody
- CENPA / CID antibody
see all
图片
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Drosophila S2 cells treated with ab10887 at 1:100 dilution and fixed with 3% PFA for 3 mins. Red = DAPI Green = ab10887
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Anti-CID antibody (ab10887) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) (ab27258) at 20 µg
Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 12 kDa (possible non-specific binding), 42 kDa (possible non-specific binding)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab10887 被引用在 13 文献中.
- Popova JV et al. Genetic Control of Kinetochore-Driven Microtubule Growth in Drosophila Mitosis. Cells 11:N/A (2022). PubMed: 35883570
- Bobkov GOM et al. Spt6 is a maintenance factor for centromeric CENP-A. Nat Commun 11:2919 (2020). PubMed: 32522980
- Huang A et al. Phosphorylation of Drosophila CENP-A on serine 20 regulates protein turn-over and centromere-specific loading. Nucleic Acids Res 47:10754-10770 (2019). PubMed: 31535131
- Pavlova GA et al. RNAi-mediated depletion of the NSL complex subunits leads to abnormal chromosome segregation and defective centrosome duplication in Drosophila mitosis. PLoS Genet 15:e1008371 (2019). PubMed: 31527906
- Strunov A et al. Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors. BMC Biol 16:68 (2018). PubMed: 29907103