概述

  • 产品名称Anti-CID抗体
  • 描述
    兔多克隆抗体to CID
  • 经测试应用适用于: WB, IHC-FoFr, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Synthetic peptide derived from residues 1 - 100 of Drosophila melanogaster CID.

  • 阳性对照
    • Drosophila Embryo Lysate;

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-FoFr
ICC
ICC/IF
  • 应用说明ICC/IF: Suggested working dilution is 1/100 (see PMID: 17077127).
    IHC-FoFr: Use at an assay dependent dilution (PMID 19137063).
    WB: 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
    • 序列相似性Belongs to the histone H3 family.
    • 细胞定位Nucleus. Chromosome, centromere, kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Co-expressed with yemalpha.
    • Information by UniProt
    • 数据库链接
      • 别名
        • CENP A antibody
        • CENP-A homolog antibody
        • CENPA / CID antibody
        • CENPA antibody
        • Centromere identifier antibody
        • Centromere identifier protein antibody
        • Centromere protein A antibody
        • cid antibody
        • CID/CENP A antibody
        • CID_DROME antibody
        • Histone H3 like centromeric protein cid antibody
        • Histone H3-like centromeric protein cid antibody
        see all

      Anti-CID antibody 图像

      • Drosophila S2 cells treated with ab10887 at 1:100 dilution and fixed with 3% PFA for 3 mins. Red = DAPI Green = ab10887
      • Anti-CID antibody (ab10887) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) (ab27258) at 20 µg

        Secondary
        Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

        Performed under reducing conditions.

        Predicted band size : 26 kDa
        Observed band size : 26 kDa
        Additional bands at : 12 kDa (possible non-specific binding),42 kDa (possible non-specific binding).

      Anti-CID antibody (ab10887)参考文献

      This product has been referenced in:
      • Moshkin YM  et al. Histone Chaperone NAP1 Mediates Sister Chromatid Resolution by Counteracting Protein Phosphatase 2A. PLoS Genet 9:e1003719 (2013). Read more (PubMed: 24086141) »
      • Yan R  et al. SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in Drosophila melanogaster. J Cell Biol 188:335-49 (2010). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 20142422) »

      See all 8 Publications for this product

      Product Wall

      Abcam has not validated the combination of species/application used in this Abreview.
      Application IHC - Wholemount
      Sample Fruit fly (Drosophila melanogaster) Embryo (Drosophila embryo)
      Specification Drosophila embryo
      Username

      Dr. Jung Wan Mok

      Verified customer

      提交于 Jun 06 2016

      Thank you for your reply. Unfortunately, since the new lot is still in production, I am unable to set up an automatic alert for when it comes into stock or an order for a lot that doesn't exist yet. I have issued you a refund (Credit Note ***) and will...

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      Thank you for your reply. That is no problem, just let me know when you decide whether you would like the refund or not. If you have any further questions I would be happy to help!

      Thank you for your patience. I checked with my colleagues in our production lab and we are currently scheduled to begin immunizations for a new batch of ab10887 in one week. This would mean that a new lot would be available for sale in a couple of mont...

      Read More

      According to our records, ab10887 was showing lot-to-lot variation and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting some images to forward to the lab, and we would be happ...

      Read More
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application Immunocytochemistry
      Sample Fruit fly (Drosophila melanogaster) Cell (DM2 cells)
      Specification DM2 cells
      Fixative Paraformaldehyde
      Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1
      Username

      Dr. Beth Sullivan

      Verified customer

      提交于 Oct 05 2005

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"