antigen, corresponding to amino acids 96-185 (ACPSLKIEYA FRIYDFNENG FIDEEDLQRI ILRLLNSDDM SEDLLMDLTN HVLSESDLDN DNMLSFSEFE HAMAKSPDFM NSFRIHFWGC) of Human CIB4.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Contains 3 EF-hand domains.
Information by UniProt
- Calcium and integrin-binding family member 4 antibody
- CIB4 antibody
- CIB4_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-CIB4 antibody (ab122311)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (actin filaments). Recommended concentration of ab122311 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIB4 antibody (ab122311)
ab122311, at 1/500, staining CIB4 in paraffin embedded Human liver tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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