The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Use at an assay dependent dilution.
May convert the inactive conformation of integrin alpha-IIb/beta3 to an active form through binding to the integrin cytoplasmic domain. Induces cell migration and spreading mediated through integrin (possibly via focal adhesion complexes). Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. May play a role in regulation of apoptosis. Interacts with and up-regulates PTK2 activity. Down regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling.
Contains 2 EF-hand domains.
Membrane. Cytoplasm. Nucleus. Cell projection > filopodium. Apical cell membrane. Localizes to the perinuclear region in the presence of NBR1. Colocalizes with TAS1R2 in apical regions of taste receptor cells.